FastQCFastQC Report
Sat 16 Sept 2023
SRR15446785_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446785_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18357653
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA3963882.159252056894201No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1693020.9222420752805383No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1588670.8653992969580588No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC1530570.8337503710305452No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC1443840.786505769555618No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG1141220.6216589887607092No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA1111500.6054695553946902No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC987290.5378084006708265No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC951250.5181762614207819No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT929640.5064046041179665No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT852930.46461821671866227No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC844940.46026580848869947No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC833690.45413757412235645No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT801840.4367878617163098No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA783950.42704260724396526Illumina PCR Primer Index 6 (96% over 29bp)
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA776510.42298980158302374No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC756410.41204068951515754No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC734360.40002935015712515No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC695140.37866496332619426No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT692130.3770253201757327No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA679330.3700527512966935No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA646460.35214741230809843No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT639520.34836697261899435No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT631430.34396009119466414No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC610510.3325642989329845No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA595570.324426003694481No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC593900.32351630134854387No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT590000.32139184676821164No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA589040.3208689041022837No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC526600.2868558415392207No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC509790.27769889756604504No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA501580.2732266483084739No Hit
ACCTGCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTC488940.2663412365404227No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT458610.2498195166887619No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA451310.2458429734999349No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT429130.23376081898922482No Hit
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG424800.23140212967311236No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA424240.23109707978465438No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT420440.22902709839868965No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA392890.2140197333504452No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA371330.20227531264481358No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT368330.20064111681378877No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA365530.19911586737149894No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT354960.1933580507268549No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA332850.1813140274522021No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA330580.18007748594005998No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC323130.17601923295968172No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA321320.1750332681416301No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATAT311100.16946610767727227No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA309320.16849648481753085No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT302770.16492849058646006No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA300380.16362658124107696No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC298990.1628694038393688No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT297300.16194880685455815Illumina PCR Primer Index 6 (95% over 21bp)
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT294660.16051071452325633No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCA289280.15758005666628516No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA284290.15486184426734723No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC275740.15020438614892656No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC274510.14953436585820637No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA271010.14762780405534412No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA265920.1448551184620387No Hit
AAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATA262850.14318279139495665No Hit
TTGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC259870.14155949020280534No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA256230.13957666592782858No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA251500.13700008383424614No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC251380.13693471600100512No Hit
AAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA248890.13557833346125456No Hit
GCCCGGATAGCTCAGTCGGTAGAGCAGGGGCTGTAGGCACCATCAATATC247500.1348211560595464No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT243900.13286012106231662No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT235000.12801200676360971No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT234890.12795208624980547No Hit
ACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGCTGTAGGCACCA234850.12793029697205846No Hit
ATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATC233280.12707506782048883No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC230920.12578950043341597No Hit
ACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAA227040.12367594049195724No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT223240.12160595910599248No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATCTC221060.12041844346878113No Hit
TGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCACC213330.11620766554417386No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC211840.11539601494809822No Hit
GAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT208070.11334237552044371No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATA204670.11149028691194894No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGTC201770.10991056427529161No Hit
AACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCA200120.10901175656822797No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT195480.10648420034957629No Hit
ATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACC193460.1053838418233529No Hit
CGGTGATTGGCGCAGCCTGGTAGCGCACTTCGCTGTAGGCACCATCAATA185670.10114037998212516No Hit
ACCTGCGGTTGGATCACCTGTAGGCACCATCAATATCTCGTATGCCGTCT185300.10093882916296544Illumina PCR Primer Index 6 (95% over 21bp)
ACCTGCGGTTGGATCACCTCCCTGTAGGCACCATCAATATCTCGTATGCC183630.1000291268170283No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)