FastQCFastQC Report
Sat 16 Sept 2023
SRR15446786_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446786_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18010432
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT7114363.950132900754407No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG6947803.8576531645659586No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT6151093.415292870265411No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT4037652.241839618283448No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC3542511.9669211710191072No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATA2704491.501624169814472No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA1750430.9718978423171638No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1660540.9219878790247784No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA1647890.9149641718754996No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1534750.8521450235063767No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA1474430.818653322696535No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1409940.7828462970793815No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA1212380.6731543141219489No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC1062730.590063580929097No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC981480.5449508373813576No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT927610.5150403943669979No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCA880600.48893885499248435No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT819170.45483084470155966No Hit
AGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATG789900.43857915234903855No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC777460.43167204429077555No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG774560.43006186636722543No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCCTGTAGGC766420.4255422635059503No Hit
AAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA762740.423499003244342No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT746470.41446534985945926No Hit
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG743010.412544241026534No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATA691760.3840885104810368No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT682540.378969255151681No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC650690.3612850596809671No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATA632420.3511409387626016No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC564590.31347943236453185No Hit
TGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCA561210.3116027422329459No Hit
GGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGC521920.2897876075376759No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC514820.28584544779381194No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC513170.2849293120786886No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG487560.2707097753124411No Hit
AAGGCTGAGGCGTGATGACGAGGCTGTAGGCACCATCAATATCTCGTATG486550.2701489892080323No Hit
ATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGCC466280.258894400756184No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT449610.24963865386460468No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC423460.23511929086431688No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC391130.21716858318556712No Hit
GAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT384010.21321531876636832No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA379750.2108500229200499No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGCTGTAGGCAC378070.20991723019192435No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT369010.20488681226524716No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC361250.20057819823533382No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC354480.1968192656344945No Hit
CAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT346730.19251620394224858No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCCTGTAGGCA342300.19005651835558415No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT320640.17803015496796523No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATAT308670.17138400678007057No Hit
AGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTA304560.16910199599876338No Hit
TAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGT299840.16648129261974393No Hit
TAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA296690.1647323062545085No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA295180.16389390326672898No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA289090.1605125296272738No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT288910.16041258754926035No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC281530.15631496235070874No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT279460.15516562845355403No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA274570.15245053533418854No Hit
CTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC269130.14943006364311529No Hit
GACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT268620.14914689442207715No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA262020.14548235156158387No Hit
CTTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGT257710.14308929402692838No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA256760.14256182194852404No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT253930.14099051038864585No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT250540.1391082679193925No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA244520.1357657606436092Illumina PCR Primer Index 6 (96% over 29bp)
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA238340.13233441596514733No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA238310.1323177589521451No Hit
GATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC233790.1298081023264739No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA230280.12785923180521155No Hit
ACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAA228540.1268931250510815No Hit
CTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA227300.12620463518032216No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATATCTC225530.12522187141318986No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA222470.12352285608696115No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA221580.1230286980345613No Hit
CACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT220110.12221250439745143No Hit
GGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTAT215850.11984720855113304No Hit
AACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCA214410.11904767192702541No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA213150.11834807738093121No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC208630.11583842075526007No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATC206660.11474461023477948No Hit
AAGGCTGAGGCGTGATGACGAGCTGTAGGCACCATCAATATCTCGTATGC205010.11382847451965616No Hit
GAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT203870.11319550802557096No Hit
TTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGC202370.11236265737545885No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT201500.11187960399839383TruSeq Adapter, Index 6 (95% over 22bp)
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA200070.1110856197119536No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATA199670.11086352620525704No Hit
CAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT193040.1071823263317615No Hit
AAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGT188970.10492252490112398No Hit
CATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC188670.10475595477110154No Hit
GGGGCTGATTCTGGATTCGACGGGCTGTAGGCACCATCAATATCTCGTAT183770.10203530931406865No Hit
GAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA183000.10160777931367776No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCCTGTAGGC182290.10121356333929135No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)