FastQCFastQC Report
Sat 16 Sept 2023
SRR15446787_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446787_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22176908
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG3790211.7090795524786415No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA2618131.1805658390249896No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA2264141.0209448494803695No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT2075850.9360412190914982No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT1649350.7437240574745586TruSeq Adapter, Index 6 (96% over 27bp)
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC1550960.699358089053713No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1505910.6790441661209038No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT1489210.6715138106718934No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1385360.6246858218467606Illumina PCR Primer Index 6 (96% over 29bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC1327080.5984062340881785No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC1268510.5719958796780867No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC1201510.5417842739844527No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1189690.5364544056367101No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC1186910.5352008494601682No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT1066260.4807974132372285No Hit
ACCTGCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTC1030550.4646950783220095No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT888050.40043905128704144No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC828950.37378970954832835No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT816550.36819830789756625No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC811140.3657588334676773No Hit
AACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCA737500.3325531223739576No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT707800.3191608135814064No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT702140.3166086092795263No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA700790.3159998679707739No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT681700.30739181494552803No Hit
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG666330.3004611824155108No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA648190.2922815029038313No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC642250.2896030411453211No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT600080.27058776633785014No Hit
AAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATA596480.2689644561811773No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC594310.26798596089229393No Hit
ATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATC556580.25097276861138623No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA554050.24983194230683556No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC552240.24901577803361946No Hit
ACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAA551430.24865053324836808No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA543060.24487633713410364No Hit
TGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCT534050.2408135525475418No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA518530.2338152820943298No Hit
GGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT514800.23213335240422156TruSeq Adapter, Index 6 (95% over 22bp)
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT495530.22344413387114198No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC482950.21777156671254622RNA PCR Primer, Index 6 (96% over 26bp)
AAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC471370.21254991904191511TruSeq Adapter, Index 6 (95% over 23bp)
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC469200.21157142375303178No Hit
ACCTGCGGTTGGATCACCTGTAGGCACCATCAATATCTCGTATGCCGTCT463790.20913194932314277Illumina PCR Primer Index 6 (95% over 21bp)
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA458400.2067014932830131No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC453880.20466333719741273No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA442860.19969420444004185No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA436970.19703828865592984No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA435800.19651071285501118No Hit
TTGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATC428710.19331369368534151No Hit
CTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC424360.19135219391269515No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA411350.18548573137427454No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT408430.18416904646941765No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC402860.18165742492145434No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC393750.17754954838609602No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA392210.1768551323746304No Hit
GAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC388800.1753174969206708Illumina PCR Primer Index 6 (100% over 22bp)
TAACCGTGAGGCTTAACCCTGTAGGCACCATCAATATCTCGTATGCCGTC387280.1746320992989645No Hit
ACGATGTCGACTTGGAGGTTGTGCCTGTAGGCACCATCAATATCTCGTAT376260.16966296654159363No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA366530.1652755199236972No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA366320.16518082683122462No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGCTGTAGGCACCATCAATATCTC352390.15889951836387653No Hit
TGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCA349620.15765047138221433No Hit
GGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGC349210.1574655943921488No Hit
TGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC346280.15614440029241228TruSeq Adapter, Index 6 (96% over 26bp)
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA341990.15420995568904375No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAA341380.1539348948013853No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT336100.15155403990493174No Hit
CCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGT334840.15098588135009625No Hit
TCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCG321270.14486690389841542No Hit
AAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACC320970.14473162805202602No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT319760.14418601547158874No Hit
CTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA317510.14317144662366818No Hit
CTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT314530.14182770654953342RNA PCR Primer, Index 6 (96% over 28bp)
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC309150.1394017597042834No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATCTC304460.137286947305729No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT304240.13718774501837677No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA295330.13317005238061141No Hit
CAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT293330.13226821340468203No Hit
GCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATG288020.12987383092358953No Hit
TGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT286040.12898101033741943Illumina PCR Primer Index 6 (100% over 23bp)
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA282510.1273892645449041No Hit
TGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTAT282320.1273035898421908No Hit
TGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG278640.12564420612648075TruSeq Adapter, Index 6 (96% over 29bp)
GATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT276980.12489567977645936RNA PCR Primer, Index 6 (96% over 27bp)
GAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT270340.12190157437637385No Hit
AGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT268840.12122519514442681RNA PCR Primer, Index 6 (95% over 24bp)
TGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCACC268210.12094111586700906No Hit
AAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT266420.12013396998355227TruSeq Adapter, Index 6 (95% over 24bp)
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC264580.11930427812569724No Hit
AAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC262680.11844753109856432RNA PCR Primer, Index 6 (96% over 26bp)
ACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGCTGTAGGCACCA262590.11840694834464749No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC262420.11833029203169351No Hit
GTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGA262210.11823559893922092Illumina PCR Primer Index 6 (100% over 28bp)
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA261000.11768998635878365No Hit
ATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACC259800.11714888297322601No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC256250.11554811879095138No Hit
TCCTGCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTC253180.11416379596289979No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA243020.10958245396517854No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCA240320.1083649713476739No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCA239340.10792307024946851No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCCTGTAGGCAC237850.10725120021240112No Hit
AGGTGGGAGGCTTTGAAGTGTGGACCTGTAGGCACCATCAATATCTCGTA237020.10687693703739043No Hit
TAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCAC236160.10648914627774078No Hit
GAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT234520.1057496383174787No Hit
GTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG234010.10551966937861672RNA PCR Primer, Index 6 (96% over 25bp)
CACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC232990.10505973150089273RNA PCR Primer, Index 6 (96% over 26bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT230960.1041443649403244No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT228080.10284571681498612No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATA225920.10187173072098239No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT224540.10124946182759112No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTGTAGGCACCATCAATA223620.10083461589866359No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCTGTAGGCACCATCAA222290.10023489297967057No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)