FastQCFastQC Report
Sat 16 Sept 2023
SRR15446788_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446788_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15826577
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA2543981.6074101178037425No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA1628431.0289211621691792No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA1350410.8532546235360937No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT1115040.704536426291042No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT1011180.6389126341090685No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC995480.6289926116051501No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC869790.54957556520276No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT829920.5243837628313437No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT829830.5243268964603022No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA814690.5147607091539756No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC661280.4178288204707815No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT656840.41502341283273064No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA655360.4140882769533804No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC651190.41145346842845426No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA643850.4068156999457305No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA630870.3986143055444017Illumina PCR Primer Index 6 (96% over 29bp)
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA592610.37443977936606254No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT588840.37205771026798784No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT570080.36020423114865585No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA490770.3100923212896889No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC464890.2937400803724014No Hit
CGGTGATTGGCGCAGCCTGGTAGCGCACTTCGCTGTAGGCACCATCAATA448380.28330826052910874No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA439200.27750789068286846No Hit
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG425340.26875046954246645No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT420830.2659008325047166No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC412950.26092186579574345No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA411470.25998672991639316No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT411180.25980349383192586No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT406280.2567074358529959No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAT387720.2449803264470896No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT366280.23143349316785303No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG358460.2264924373729076No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC350490.22145660429289288No Hit
GGGTCGTTAGCTCAGTTGGTAGAGCCTGTAGGCACCATCAATATCTCGTA340640.21523289590667646No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC332820.21029184011173105No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA331880.20969790245863018No Hit
GGGGGCTGATTCTGGATTCGACGGGCTGTAGGCACCATCAATATCTCGTA293820.18564974599371678No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT274930.17371412656065804No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC256730.16221448263891808No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT254370.16072332002049464No Hit
TGGGGCTGATTCTGGATTCGACGGGCTGTAGGCACCATCAATATCTCGTA251700.15903628434626135No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA251620.15898573646089106No Hit
ACCGAGCGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATAT249200.15745666292843993No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC248790.1571976050159172No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCA226580.14316424833999167No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA226450.14308210802626492No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT215820.13636555775768822No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA214990.1358411234469715No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA214470.13551256219206465No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGG213270.13475434391151037No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA212470.1342488650578075No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC210830.13321263340771664No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCTGTAGGCACCAT201150.12709633927791208No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA201020.12701419896418537No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCCTGTAGGCACCATCAATA200920.12695101410747253No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA200700.1268120074227042No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTGTAGGCACCATCAATATCTCG198280.12528293389025308No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT196540.12418351738344936No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATC183190.11574833901228294No Hit
CGGTGGTTCGAGTCCACTCGGACGCCTGTAGGCACCATCAATATCTCGTA182100.1150596240741128No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT176840.11173610061101652No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATATCTCGTA169860.10732579761245908No Hit
GGGTCGTTAGCTCAGTTGGTAGAGCTGTAGGCACCATCAATATCTCGTAT169350.10700355484322352No Hit
TGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCCTGTAGGCACCATCAA168700.10659285327458995No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC166950.10548711828211495No Hit
GGCTACGTAGCTCAGTTGGTTAGAGCCTGTAGGCACCATCAATATCTCGT166650.10529756371197639No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA159660.10088094222774767No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)