FastQCFastQC Report
Sat 16 Sept 2023
SRR15446790_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446790_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18133483
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA2413361.3308860741204545No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT2140041.1801593769933774TruSeq Adapter, Index 6 (96% over 27bp)
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC2070711.1419262366749952No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT1906441.0513369108405703No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1808810.997497281686039Illumina PCR Primer Index 6 (96% over 29bp)
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA1379770.7608962933375788No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC1247620.6880200565991652No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1174530.6477134039831179No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT1031750.5689750832755075No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT975410.5379054867727286No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC971090.5355231534945603No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA954490.5263688172867838No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC951770.5248688296671964No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA905400.4992973495494495No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT904960.4990547044933398No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC889320.4904297756807117No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT878820.48463938229627485No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA757590.4177851546776756No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT729410.40224484176591996No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC675440.372482219769914RNA PCR Primer, Index 6 (96% over 26bp)
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG670770.36990687337893113No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC658010.36287016675174866No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT657960.36284259344991804No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA639400.3526073838103799No Hit
TGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT623080.3436074580928551Illumina PCR Primer Index 6 (100% over 23bp)
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA605370.33384099458443806No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC580370.320054343669112No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA578160.31883560372819714No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC528840.2916372988024419No Hit
CGGTGATTGGCGCAGCCTGGTAGCGCACTTCGCTGTAGGCACCATCAATA516710.2849480157783257No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA482520.2660933919865257No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA459740.25353099567248055No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT439730.24249616027985357No Hit
GTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGA423720.23366718903367875Illumina PCR Primer Index 6 (100% over 28bp)
GAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT422990.23326461882695124No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA408560.22530696391862504No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT402420.22192096245382092No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT396250.21851841700791844No Hit
CTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT382840.21112325745693752RNA PCR Primer, Index 6 (96% over 28bp)
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA378020.20846519116046267No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGCTGTAGGCACCATCAATATCTC371080.20463801686636815No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC365140.20136230860888665No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA354340.1954064754134658No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGCTGTAGGCACCATCAAT353380.19487706801831728No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT330220.1821051146103592No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA325570.17954079754010854No Hit
GCCCGGATAGCTCAGTCGGTAGAGCAGGGGCTGTAGGCACCATCAATATC311570.17182027302752595No Hit
CCTTGGTTCGATTCCGAGTCCGGGCCTGTAGGCACCATCAATATCTCGTA303370.167298251527299No Hit
CAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT303150.1671769289992441No Hit
ACTGTTTCGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT301120.16605745294491964TruSeq Adapter, Index 6 (96% over 28bp)
CGGTGGTTCGAGTCCACTCGGACGCCTGTAGGCACCATCAATATCTCGTA293940.16209792680203797No Hit
AAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT291280.16063102714464728No Hit
TGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG290570.16023948625865203TruSeq Adapter, Index 6 (96% over 29bp)
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC278570.1536218938192955No Hit
AAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACC276270.1523535219350855No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT271260.14959067709165416No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA270580.1492156801867573No Hit
CACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC266240.1468223175878567RNA PCR Primer, Index 6 (96% over 26bp)
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT260730.1437837397261188No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA256250.1413131718820924No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT251730.1388205453966014No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGTAGGCACCATCAATA246260.13580402617632806No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAT244690.13493822449884557No Hit
TGCAAGAGGTCAGCGGTTCGATCCCGCTTAGCTCCCTGTAGGCACCATCA243040.13402830553843406No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATATCTCGTA237710.13108899156328654No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC236100.13020113124433955No Hit
ACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAA235930.13010738201811534No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAA235520.129881280943104No Hit
TGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCA232760.128359234682052No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA231420.1276202701929905No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCA228660.1260982239319385No Hit
AGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT226660.12499529185871243RNA PCR Primer, Index 6 (95% over 24bp)
ACTTCGAAGGTTCGAATCCTTCCCCCACCACCACTGTAGGCACCATCAAT221020.12188502341221485No Hit
GGAAGTGTGGCCGAGCGGTTGAAGGCACCGGCTGTAGGCACCATCAATAT216140.1191938691535432No Hit
AACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCA214850.11848247796631237No Hit
TGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC214320.11819020096690747TruSeq Adapter, Index 6 (96% over 26bp)
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA213910.1179640998918961No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCTGTAGGCACCATCAATATC211380.11656889081926511No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT209710.11564794253812133No Hit
TTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCAAT207960.1146828769740485No Hit
ACCGAGCGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATAT204740.1129071563361545No Hit
TAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCAC201600.11117555298118954No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCTGTAGGCACCATCAA201290.1110045985098395No Hit
TGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCCTGTAGGCACCATCAA201230.11097151054764272No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTGTAGGCACCATCAATA199950.11026563402077802No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC199240.10987409313478276No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC199180.10984100517258599No Hit
GATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT199010.10974725594636177RNA PCR Primer, Index 6 (96% over 27bp)
TGGGGTATCGCCAAGCGGTAAGGCACCGGCTGTAGGCACCATCAATATCT196050.10811491647798716No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT195570.10785021278041289No Hit
GGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGC194830.10744212791331924No Hit
GTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCCTGTAGGCACCATCAAT192370.10608552146325115No Hit
CTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC189900.10472340035281695No Hit
TGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA186730.10297525301675359No Hit
CGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCATC184410.10169585181181134No Hit
GGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATA184070.10150835335936288No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCAGGGGCTGTAGGCACCATCAATAT183740.1013263695672806No Hit
TGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATA182970.10090174071908854No Hit
TGAGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT182720.10076387420993528Illumina PCR Primer Index 6 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)