FastQCFastQC Report
Sat 16 Sept 2023
SRR15446793_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446793_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19691332
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1871900.950621319065668Illumina PCR Primer Index 6 (96% over 29bp)
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA1711850.8693419012995159No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC1691090.8587991914411884No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT1554000.7891797263892559No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1268700.644293641486518No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT1242330.6309019623456656No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC1133420.5755933626023877No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA1116960.5672343546896675No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT1032040.5241087804522314TruSeq Adapter, Index 6 (96% over 27bp)
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC1018690.5173291476676134No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG1011220.5135356003341979No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC941540.4781494720621236No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT837620.4253749822510737No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT789190.40078040429159395No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT785060.39868303474848726No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT736310.37392594873724133No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT732860.3721739088041378No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC715860.3635406685540623No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA683990.3473558822734795No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC663020.3367065265061805No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA633560.3217456289904614No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC633140.32153233717251833No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA586370.2977807697315753No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC575550.2922859662312331No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT544120.27632462852182876No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA498110.2529590177038303No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC492090.24990183497998003RNA PCR Primer, Index 6 (96% over 26bp)
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT474900.24117210557416838No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC442910.22492637877417335No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA441320.22411891689196037No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC439640.2232657496201882No Hit
AACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCA434410.22060975864913557No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT423890.21526730644732414No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT400280.20327725925295453No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA392680.199417693023509No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC390490.1983055285442346No Hit
ACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAA379260.19260251160256706No Hit
TGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCT377810.1918661469930018No Hit
AAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATA368690.18723466751766718No Hit
ATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATC367350.1865541650508965No Hit
GAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT355060.18031284018775368No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCCTGTAGGCAC349930.17770763298287795No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA339340.17232963214474267No Hit
TGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT336220.17074517864002292Illumina PCR Primer Index 6 (100% over 23bp)
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC333670.16945019260251162No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA328390.1667688097483705No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA323000.1640315647514348No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA314680.1598063554055155No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA314090.1595067311850717No Hit
TTGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATC314020.15947118254874784No Hit
CTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC308650.15674409430504752No Hit
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG307580.15620070800695454No Hit
AAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC306970.15589092703327537TruSeq Adapter, Index 6 (95% over 23bp)
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA305180.15498189761870856No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA299680.15218879047897826No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC292370.1484764971714458No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAA290140.1473440191857006No Hit
TAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCAC287790.1461506006805431No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGCTGTAGGCACCATCAATATCTC280750.14257542354168828No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA269320.13677083906766693No Hit
CTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT258730.13139283822953166RNA PCR Primer, Index 6 (96% over 28bp)
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA257260.130646316866731No Hit
AAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT256240.13012832245172648No Hit
TGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC255950.12998104952981343TruSeq Adapter, Index 6 (96% over 26bp)
ACTTCGAAGGTTCGAATCCTTCCCCCACCACCACTGTAGGCACCATCAAT254270.12912788225804125No Hit
TAACCGTGAGGCTTAACCCTGTAGGCACCATCAATATCTCGTATGCCGTC246870.12536988356094955No Hit
AGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT245500.12467414596432583RNA PCR Primer, Index 6 (95% over 24bp)
GGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT244890.12436436499064665TruSeq Adapter, Index 6 (95% over 22bp)
ACCTGCGGTTGGATCACCTGTAGGCACCATCAATATCTCGTATGCCGTCT241870.12283069525210381Illumina PCR Primer Index 6 (95% over 21bp)
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA239660.121708374019594No Hit
CAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT232020.11782849428367771No Hit
CCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGT231680.11765582947867619No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT228940.11626435428542875No Hit
AAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACC225190.11435996305379442No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA224670.11409588746967447No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT221510.11249112045848397No Hit
AGGTGGGAGGCTTTGAAGTGTGGACCTGTAGGCACCATCAATATCTCGTA221000.11223212325098171No Hit
GCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATG219970.11170905045935949No Hit
TGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG218940.11118597766773726TruSeq Adapter, Index 6 (96% over 29bp)
GATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT218800.11111488039508959RNA PCR Primer, Index 6 (96% over 27bp)
GAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC217510.11045976981140739Illumina PCR Primer Index 6 (100% over 22bp)
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA215710.10954566202022291No Hit
TGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTAT214130.10874327851462764No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT213130.10823544085285851No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC212590.10796120851550317No Hit
GGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGC210830.10706741423078946No Hit
CACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC210150.10672208462078644RNA PCR Primer, Index 6 (96% over 26bp)
AAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA209230.10625487397195883No Hit
GAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT199600.10136439728912193No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)