FastQCFastQC Report
Sat 16 Sept 2023
SRR15446795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20983768
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT7624693.6336133720121193No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT6883223.2802592937550585No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT4542212.164630299000637No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG2427621.1569037553217325No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2309231.100483955026571Illumina PCR Primer Index 6 (96% over 29bp)
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA2302911.097472103199006No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC2214891.0555253946764946No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT2169041.0336751721616442No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA2050620.9772410751014784No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1886210.8988900372897756No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT1789790.8529402345660704No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA1772120.8445194399785586No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1586700.7561559010755361No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATA1481240.7058980064972126No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT1420260.676837448831878No Hit
GAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1168100.5566683733827023No Hit
GGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGC1084950.5170425063792166No Hit
AAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA1083960.5165707131340758No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC1045280.4981374174552445No Hit
CAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1043900.49747976626504825No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC923630.44016403536295295No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC844070.40224901457164414No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT816490.3891055219443905No Hit
CTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC806200.3842017315479279No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC779860.3716491718741839No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT684050.3259900700388986No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA638960.3045020322374895No Hit
AAGGCTGAGGCGTGATGACGAGGCTGTAGGCACCATCAATATCTCGTATG615760.2934458673008585No Hit
AAGGCTGAGGCGTGATGACGAGCTGTAGGCACCATCAATATCTCGTATGC572220.2726964956913363No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC564790.2691556635586135No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA525350.2503601831663408No Hit
TGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCA521390.2484730101857779No Hit
TGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATG517300.2465238845568632No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT510760.2434071897859336No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC493000.23494350490340915No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC478750.22815254152638362No Hit
GACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT477570.22759020210288256No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC476300.2269849723843687No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATA465150.2216713413911172No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG457160.21786363631164812No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC449180.21406069682051382No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCCTGTAGGCACC448780.21387007328712368No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT446660.21285976856015562No Hit
TAGGCTGAGGCGTGATGACGAGGCTGTAGGCACCATCAATATCTCGTATG438320.2088852678889702No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA433990.20682176814002137No Hit
TAGGCTGAGGCGTGATGACGAGCTGTAGGCACCATCAATATCTCGTATGC428640.20427217838092757No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC423450.20179883803518986No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA419950.20013088211802568No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCCTGTAGGCAC414820.19768613530229653No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATA402810.19196266371225607No Hit
CACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT402160.19165290047049702No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA379110.1806682193588873No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA366630.17472076511711337No Hit
GGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATG350150.16686707554143754No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATC344860.1643460793123523No Hit
TAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCAC344610.1642269396039834No Hit
AAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACC341570.16277820075021798No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATA334690.1594994759759067No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATA334300.15931361803085126No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA332930.15866073242898987No Hit
TGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT327260.15595864384318395Illumina PCR Primer Index 6 (100% over 23bp)
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT327100.15588239442982788No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC325690.15521044647462742No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT323520.15417631380598565No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT312410.14888174516607314No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAA311430.14841471750926716No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATA310390.14791909632245268No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA301010.1434489744644527No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC298170.14209554737738236No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA290900.13863096465801567No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT288910.13768261257939945TruSeq Adapter, Index 6 (95% over 22bp)
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG284340.13550473871041654No Hit
GAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT279560.1332267874864038No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC279030.13297421130466178No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT273350.13026735713052107No Hit
CTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA271290.1292856459335616No Hit
CAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA270990.12914267828351894No Hit
TAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA266920.12720308383127377No Hit
GAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA262790.12523489584902006No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATATCTC254280.12117938017614377No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT253190.12065993104765552No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA238290.11355920442887094No Hit
ACCTGCGGTTGGATCACCTGTAGGCACCATCAATATCTCGTATGCCGTCT232320.11071414819302235Illumina PCR Primer Index 6 (95% over 21bp)
TTAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA230130.10967048434771104No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA229840.10953228228600316No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGCTGTAGGCACCATCAATAT227870.10859346138405647No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA220550.105105050723016No Hit
AAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT218450.1041042771727175No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA213200.10160234329697126No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA211300.10069688151336786No Hit
GTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC210140.1001440732665363No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)