FastQCFastQC Report
Sat 16 Sept 2023
SRR15446796_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446796_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17877503
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC1679570.9394880258165808No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC1216160.6802739733843144No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA1040790.5821786185688236No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA984740.5508263654046094Illumina PCR Primer Index 6 (96% over 29bp)
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC898150.5023911896420882No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT822870.4602824007357179TruSeq Adapter, Index 6 (96% over 27bp)
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT784230.4386686440494514No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC763610.4271345948033123No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCA679040.3798293307516156No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC646100.3614039387939132No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT630630.3527506050481435No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC628150.35136338671010153No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG543290.3038959076108112No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT539370.301703207657132No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT539330.3016808331678087No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA518990.2903034053469324No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT517910.28969929413520445No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA505810.28293101111491914No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT489550.27383578120501506No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC481350.2692490108937473No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT454140.25402876453160156No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA446380.24968811360288964No Hit
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG432030.24166126555817108No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC429720.240369138799753No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA422990.23660463097111492No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC419020.23438396290578167No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC392740.21968392342040158No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT387880.21696542296762586No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT387300.21664099287243863No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATC382590.21400639675462507No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA352740.19730943409714433No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA338730.18947276921167347No Hit
ATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACC335430.18762687384250473No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC332260.18585369556363662No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA319640.17879454418214893No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC312570.1748398531942632No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA302820.16938607142171924No Hit
ACTTCGAAGGTTCGAATCCTTCCCCCACCACCACTGTAGGCACCATCAAT299540.16755136329721212No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT282530.15803661171249697No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT279930.15658226990648527No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC273940.15323169013032747No Hit
AACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCA269990.15102220930965582No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA269840.15093830497469363No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA245620.13739055168946135No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA239470.13395047395601054No Hit
ACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAA238310.13330161376563607No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC238020.13313939871804242No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC234070.1309299178973708No Hit
ACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGCTGTAGGCACCA230050.12868128172038343No Hit
TGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT224000.12529714021024077Illumina PCR Primer Index 6 (100% over 23bp)
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT223750.1251572996519704No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA220510.12334496601678656No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC219580.12282475914002083RNA PCR Primer, Index 6 (96% over 26bp)
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT217020.12139279182333236No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA216210.12093970841453641No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATCTC215910.12077189974461199No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT214030.11972029874641889No Hit
AAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATA209970.11744928808010828No Hit
TGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCA206880.11572085877988665No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA204950.1146412896700395No Hit
TAACCGTGAGGCTTAACCCTGTAGGCACCATCAATATCTCGTATGCCGTC199030.1113298652501974No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA195080.10912038442952576No Hit
TGGGGTATCGCCAAGCGGTAAGGCACCGGCTGTAGGCACCATCAATATCT195010.10908122907321005No Hit
AGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT194520.10880714157900015RNA PCR Primer, Index 6 (95% over 24bp)
GCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG192790.10783944491576927TruSeq Adapter, Index 6 (96% over 25bp)
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAA182480.10207242029269972No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC180240.1008194488905973No Hit
TATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCAC179700.10051739328473333No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)