FastQCFastQC Report
Sat 16 Sept 2023
SRR15446797_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446797_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14493935
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTC5170893.5676232851879077No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT2911082.0084814786322696No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC2910962.0083986853811613No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC2327121.6055819209897106No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA2206671.5224781951899191No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA1913931.3205040591116215No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC1725531.1905186548718483No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG1648541.1373998848483866No Hit
ACCTGCGGTTGGATCACCTGTAGGCACCATCAATATCTCGTATGCCGTCT1641061.1322391055293126Illumina PCR Primer Index 6 (95% over 21bp)
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT1606691.1085257385244243No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA1511761.0430293774602963No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1471481.015238442838332No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC1378490.9510805726671191No Hit
TCCTGCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTC1262910.8713368729747993No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC1227490.8468990650227147No Hit
TAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCAC1208800.8340040161626224No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1096780.7567165162531776No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT995960.6871563864471588No Hit
AAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACC904350.6239506386636893No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC858650.5924202088666742No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC834000.5754130952015446No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA833580.5751233188226661Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA806760.5566190271999978No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA787340.5432203193956645No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC757730.5227910846847319No Hit
ACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAA740320.5107791638364598No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATA625760.4317392067785595No Hit
ATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATC611370.42181091608317545No Hit
AGATCGGAAGAGCGTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC573960.3960001200502141TruSeq Adapter, Index 6 (95% over 23bp)
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT568400.3921640327488705No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC566610.39092903341984075No Hit
AAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATA565760.3903425812244915No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAA537500.3708447705885255No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA530570.36606346033703063No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT489380.33764467689416294No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA461820.31862982688966107No Hit
AACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCA454500.31357943857206483No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT442810.3055139960266139No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC440020.30358905293834976No Hit
TCCTGCGGTTGGATCACCTGTAGGCACCATCAATATCTCGTATGCCGTCT439300.3030922934317009Illumina PCR Primer Index 6 (95% over 21bp)
TAACCGTGAGGCTTAACCCTGTAGGCACCATCAATATCTCGTATGCCGTC436930.3014571267223152No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC431400.29764173773374863No Hit
AACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCCTGTAGGCACCA430670.2971380787895075No Hit
AAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT406010.2801240656867855No Hit
AAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC389380.26865030097071635TruSeq Adapter, Index 6 (95% over 23bp)
TTGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATC354100.24430908514492441No Hit
TGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCA347440.23971405970842288No Hit
AAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCAC341830.23584347521911753No Hit
ACGATGTCGACTTGGAGGTTGTGCCTGTAGGCACCATCAATATCTCGTAT337850.23309749905736435No Hit
AAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC330970.2283506859938312No Hit
ATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACC329340.22722607766627903No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC325200.22436971050304833No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT321420.22176172309314204No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA314100.21671133477554577No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA303350.2092944393637753No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT299660.2067485468922001No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA283940.19590263099703428No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC265140.18293168832342632No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC258150.1781089814463774No Hit
CTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC255880.17654280911291517No Hit
TAACCGTGAGGCTTAACCTGTAGGCACCATCAATATCTCGTATGCCGTCT244240.16851186375542598Illumina PCR Primer Index 6 (95% over 21bp)
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC243360.16790471324729964No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA240420.1658762785951503No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC236970.1634959726257914No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA229380.1582592994932018No Hit
TTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTA225370.15549262501867162No Hit
AAAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAA220410.15207050397286864No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATCTC214880.14825511498430205No Hit
TCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCG211800.1461300882058599No Hit
ACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGCTGTAGGCACCA208970.14417754736722638No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGCTGTAGGCACCATCAAT193200.13329713428409884No Hit
AACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTGTAGGCACCAT186020.12834333809279538No Hit
TCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCCTGTAGGC178580.12321015652409094No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCCTGTAGGCACCAT177670.12258230770318757No Hit
TTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCA177280.12231322963708613No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGG175650.12118862130953395No Hit
TCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGT175620.12116792299675691No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCTGTAGGCACCATCAA175020.12075395674121625No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCTGTAGGCACCA174380.1203123927353062No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCCTGTAGGCAC172780.11920848272053103No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT170770.11782169576446977Illumina PCR Primer Index 6 (95% over 21bp)
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCTGTAGGCAC170120.11737323232096734No Hit
AAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT169010.1166073947482171TruSeq Adapter, Index 6 (95% over 24bp)
AAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA166230.11468935109754529No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT165440.11414429552775007No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA164310.11336465907981512No Hit
ACCTGCGGTTGGATCACTGTAGGCACCATCAATATCTCGTATGCCGTCTT162230.11192957606060742TruSeq Adapter, Index 6 (95% over 22bp)
TAGGCTGAGGCGTGATGACGAGCTGTAGGCACCATCAATATCTCGTATGC157780.10885932633201406No Hit
ATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGCTGTAGGCACCA155520.10730005343614415No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC155300.10714826580911258No Hit
CAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT149960.10346396613480052No Hit
ACCCCGTGAACCTTTACTATAGCTTGACACTGAACACTGTAGGCACCATC149750.10331907794536128No Hit
CCTGCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT147900.10204268199077753Illumina PCR Primer Index 6 (95% over 21bp)
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT147830.1019943859276311No Hit
TGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTAT147740.10193229098930001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)