FastQCFastQC Report
Sat 16 Sept 2023
SRR15446799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16257361
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA6965764.284680644047949Illumina PCR Primer Index 6 (96% over 29bp)
ACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT2229451.3713480312087551TruSeq Adapter, Index 6 (96% over 27bp)
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC1605620.9876264665587484No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG1484180.9129279961243403No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT1241820.7638509103660797No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC1139660.7010116832615084No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA1129770.6949282851011305No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1039690.6395195382571623No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT959380.5901203768557517No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC829680.5103411310113616No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC814820.5012006561212488No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA723680.4451398969365323No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT709270.43627621973824654No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC675460.41547948649230343No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT645820.3972477451906247No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC636050.39123815974806736No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA622170.3827004887201558No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT616900.37945888019586943No Hit
AAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACC598300.3680179089336824No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT559530.3441702500178227No Hit
TGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCA558170.343333705882523No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC544260.33477758167515625No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAA527540.3244930096588247No Hit
AACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCA527010.32416700348845057No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT461210.2836930298835094No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC438120.2694902327628697No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC435000.2675711020995351No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT414210.2547830487371229No Hit
ACCTGCGGTTGGATCACCTGTAGGCACCATCAATATCTCGTATGCCGTCT414090.2547092360193023Illumina PCR Primer Index 6 (95% over 21bp)
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC405980.249720726506596No Hit
AAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATA399070.24547034417209534No Hit
TGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCT383380.23581933131705696No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA382690.2353949081895887No Hit
ATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATC382040.234995089301394No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC378020.23252236325440517RNA PCR Primer, Index 6 (96% over 26bp)
GATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT372290.22899780597847338RNA PCR Primer, Index 6 (96% over 27bp)
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATCTC365420.22477202788324624No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA359830.22133358544477177No Hit
ACCTGCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTC355240.21851024898813529No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA353390.21737230292173498No Hit
ACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAA338150.20799808775852366No Hit
CTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT336150.20676787579484765RNA PCR Primer, Index 6 (96% over 28bp)
TGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG330460.20326792275818933TruSeq Adapter, Index 6 (96% over 29bp)
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG319790.19670474193197776No Hit
TGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC318040.19562830646376125TruSeq Adapter, Index 6 (96% over 26bp)
CTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC317450.1952653939344768No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCA314180.19325399737386653No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC310640.19107652219815996No Hit
ACGATGTCGACTTGGAGGTTGTGCCTGTAGGCACCATCAATATCTCGTAT305450.18788412215242067No Hit
GTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGA305250.18776110095605308Illumina PCR Primer Index 6 (100% over 28bp)
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT300760.1849992750976004No Hit
ACTGTTTCGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT291750.1794571702012399TruSeq Adapter, Index 6 (96% over 28bp)
GGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT290400.1786267771257586TruSeq Adapter, Index 6 (95% over 22bp)
TAACCGTGAGGCTTAACCCTGTAGGCACCATCAATATCTCGTATGCCGTC284660.1750960687900084No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC274120.16861285174143578No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA273420.16818227755414916No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC271600.16706278466720398No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA265170.16310765320398557No Hit
GTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG259450.15958924698787214RNA PCR Primer, Index 6 (96% over 25bp)
GGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGC257780.15856201999820266No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATA256620.15784849705927056No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGCTGTAGGCACCATCAATATCTC245900.15125456093396708No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA243410.14972294703919042No Hit
AGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT240030.14764388882057797RNA PCR Primer, Index 6 (95% over 24bp)
CCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGT238120.14646903639526737No Hit
GAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC237600.14614918128471158Illumina PCR Primer Index 6 (100% over 22bp)
AAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC228010.14025031491888507TruSeq Adapter, Index 6 (95% over 23bp)
TTGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATC226280.1391861815703053No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT223980.13777143781207785No Hit
CCTGCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT213840.13153426315624042Illumina PCR Primer Index 6 (95% over 21bp)
ATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT209650.12895696909233917TruSeq Adapter, Index 6 (96% over 28bp)
TGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT209050.12858790550323634Illumina PCR Primer Index 6 (100% over 23bp)
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA206390.12695172359154724No Hit
CAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT203710.12530323956022135No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA203640.1252601821414927No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA202090.12430676786964379No Hit
AGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATG194030.1193490136560294No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC193480.11901070536601852No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC192540.11843250574309079No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT189350.11647031766102753No Hit
CAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT188330.11584290955955275No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT188240.11578755002118733No Hit
TGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCACC187070.11506787602243686No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT185550.11413291493004307TruSeq Adapter, Index 6 (95% over 22bp)
GCCCGGATAGCTCAGTCGGTAGAGCAGGGGCTGTAGGCACCATCAATATC182870.11248443089871721No Hit
GAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT181080.11138339119122717No Hit
CCTGCGGTTGGATCACCTGTAGGCACCATCAATATCTCGTATGCCGTCTT180740.11117425515740223Illumina PCR Primer Index 6 (95% over 22bp)
TAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCAC180500.11102662972176111No Hit
AAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT178890.11003630909100191TruSeq Adapter, Index 6 (95% over 24bp)
TCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCG178780.10996864743299972No Hit
GCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATG177130.10895372256296701No Hit
GCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC174560.10737290018964334RNA PCR Primer, Index 6 (95% over 23bp)
AACCGTGAGGCTTAACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT173580.10677009632744207Illumina PCR Primer Index 6 (95% over 21bp)
TAACCGTGAGGCTTAACCTGTAGGCACCATCAATATCTCGTATGCCGTCT173460.10669628360962151Illumina PCR Primer Index 6 (95% over 21bp)
TGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTAT173370.1066409240712561No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA170650.10496783580065669No Hit
CACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC170400.1048140593051972RNA PCR Primer, Index 6 (96% over 26bp)
AGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGCTGTAG167540.10305485619714048No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA166370.10233518219839001No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT164680.10129565308908378No Hit
AAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC163830.10077281300452147RNA PCR Primer, Index 6 (96% over 26bp)
TTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATCT163660.100668244987609No Hit
AATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAT163230.10040374941541864No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)