FastQCFastQC Report
Sat 16 Sept 2023
SRR15446800_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446800_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16947122
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCGGCAAGGGGGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCT1550800.9150816286092706Illumina PCR Primer Index 6 (95% over 21bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1450530.8559152403576252Illumina PCR Primer Index 6 (96% over 29bp)
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT1105230.6521638305312253No Hit
TTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTA1003680.5922421517942693No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA992610.5857100692377148No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT959150.5659663038951392No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA944800.557498789469976No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC927200.5471135452969537No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG874240.5158634014672225No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT873200.5152497279479076No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC842230.49697523862753806No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT827180.48809467471822054No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC824280.4863834697124385No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA817320.48227657769856147No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT774510.4570156513890677No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT774490.45700384997523474No Hit
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG707580.41752221999700007No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT685300.40437544498706035No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA639970.3776275405346111No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT609900.35988411483672567No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA562070.3316610336551539No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA528600.3119113676056619No Hit
ACAAGCAGTGGGAGCACGCCTGTAGGCACCATCAATATCTCGTATGCCGT489010.28855046892327796No Hit
GATGACGAGGCACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG481500.28411903802899396Illumina PCR Primer Index 6 (96% over 25bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT461440.272282219954515No Hit
TGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT453120.26737283179999527Illumina PCR Primer Index 6 (95% over 24bp)
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT441250.2603686926901217No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA427010.25196608604103987No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA402780.2376686731823846No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT380720.22465171372460765No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATAT376640.22224422530267973No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAT372250.2196538149663406No Hit
TGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCT338550.19976843265776928No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT338290.19961501427794054TruSeq Adapter, Index 6 (95% over 22bp)
ATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACC336720.1986886032920516No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC328950.19410375401793883No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC313640.18506977172879263No Hit
CGGTGATTGGCGCAGCCTGGTAGCGCACTTCGCTGTAGGCACCATCAATA311620.18387782893166169No Hit
CTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTA291740.17214722358167953No Hit
GGGGGCTGATTCTGGATTCGACGGGCTGTAGGCACCATCAATATCTCGTA289220.17066024543872405No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA282000.16639993504501827No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA279480.16491295690206279No Hit
GTTTCGGCAAGGGGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC275330.16246416353171944Illumina PCR Primer Index 6 (100% over 22bp)
GGGTCGTTAGCTCAGTTGGTAGAGCCTGTAGGCACCATCAATATCTCGTA274780.1621396246513125No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT272800.16097128468184746No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC265630.15674047782272413No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATATCTC265440.1566283643913108No Hit
ACTGTTTCGGCAAGGGGGTCCTGTAGGCACCATCAATATCTCGTATGCCG259990.15341247912182376No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA255820.15095188433764742No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTGTAGGCACCATCAATATCTCG246930.1457061558888878No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCAT239420.1412747249946038No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA239170.14112720732169154No Hit
TGGGGCTGATTCTGGATTCGACGGGCTGTAGGCACCATCAATATCTCGTA237950.14040732107787976No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA237760.14029520764646644No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT234530.1383892793124402No Hit
TGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGT226540.1336746144861647No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA225170.13286621763860554No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCTGTAG219240.12936709843712696No Hit
ACGTACAAGCAGTGGGAGCACGCCTGTAGGCACCATCAATATCTCGTATG216710.127874219587255No Hit
TCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAG213270.12584437640798243No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC210680.12431609331661152No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC210300.1240918664537849No Hit
AGCTTGACACTGAACACTGTAGGCACCATCAATATCTCGTATGCCGTCTT208030.12275240598374167TruSeq Adapter, Index 6 (95% over 22bp)
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT207380.12236886003416982No Hit
ATCACGGGAGACACACGGCGGGTGCTGTAGGCACCATCAATATCTCGTAT205500.12125952713386969No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCA203210.11990826524999347No Hit
GGGGCTGATTCTGGATTCGACGGGCTGTAGGCACCATCAATATCTCGTAT197850.11674548634275483No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATAT194960.11504018204388923No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCTGTAG192790.1137597286430109No Hit
TTTCCCTGGTTCGAATCCAGGTACCCCAGCCACTGTAGGCACCATCAATA187400.11057924761502277No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCCTGTAGGCACCATCAAT186670.110148496010119No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT185780.10962333309455138No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA180910.10674968882622075No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCTGTAGGCACCAT180720.10663757539480746No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA180130.10628943368673455No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCCTGTAGGC179910.10615961813457177No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC179080.10566985946050308No Hit
CGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCATC178270.10519190220026739No Hit
GCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATG176230.10398815798930343No Hit
AACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCA174800.10314435690024536No Hit
CGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCACTGTAGGCACCATCAA170430.10056574797773923No Hit
AAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATA170060.1003474218218291No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)