FastQCFastQC Report
Sat 16 Sept 2023
SRR15446801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20750783
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT5824552.806906129759055No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG4029691.9419459978931881No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT3774701.819063887854256No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT2291371.10423303062829No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1684730.8118874357656769No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA1614500.7780429297535424No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC1603270.7726310857763776No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1414180.6815068135019291No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC1392950.6712758742646No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA1053400.5076434947057179No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1043590.5029159622554966No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT1043160.5027087411593095No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCCTGTAGGC1015600.48942731462229644No Hit
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG1003290.483495008357034No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC943670.4547635624159339No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATA931760.44902401996107805No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT852520.4108375091195354No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA820800.39555133895429395No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC815740.3931128767526508No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT802030.38650589715096534No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA776520.374212385142286Illumina PCR Primer Index 6 (96% over 29bp)
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC750720.36177911937106183No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT712100.3431677734763069No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT698700.33671018582768664No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA654990.315645920445508No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT644460.3105714131365549No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT638600.30774742331409854No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT622150.2998200116111281No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA605990.29203235367070246No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA602620.29040831856802707No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA575370.27727628398407905No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA563390.2715030078624021No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATA548130.2641490684953912No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT546710.2634647569684479No Hit
ACCCCGTGAACCTTTACTATAGCTTGACACTGAACACTGTAGGCACCATC527020.2539759584011842No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG523840.2524434861084519No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA519020.25012068219305267No Hit
AAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA509830.24569193364896158No Hit
TTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGC496960.23948975804913No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT492760.23746573803986093No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGCTGTAGGCAC460510.22192415582583075No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA457670.22055553277194406No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC451480.21757251280590234No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA428170.2063392017544591No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAT426410.20549104098867016No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT389420.18766520762132205No Hit
AAGGCTGAGGCGTGATGACGAGGCTGTAGGCACCATCAATATCTCGTATG368620.1776414894801801No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATAT366040.17639816290305768No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT365500.1761379317590088No Hit
CGGTGATTGGCGCAGCCTGGTAGCGCACTTCGCTGTAGGCACCATCAATA358770.17289468064891816No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA357310.17219109274093417No Hit
TTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT356540.1718200224059015No Hit
TGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCA344430.1659840980458424No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG324430.1563459075255136No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA323850.15606640000042407No Hit
TAGGCTGAGCTGTGATGACGAGGGCTGTAGGCACCATCAATATCTCGTAT322090.15521823923463515No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA319520.1539797317527729No Hit
GATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC313400.1510304454535523No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATA276550.13327207941984648No Hit
GGGTCGTTAGCTCAGTTGGTAGAGCCTGTAGGCACCATCAATATCTCGTA275180.13261186336920394No Hit
GAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT270810.1305059187405121No Hit
CATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC269640.12994208459507287No Hit
CAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT266020.12819757211089336No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC264050.12724821034464098No Hit
GGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGC262330.1264193259598927No Hit
TAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA252730.12179299451013487No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCA250800.12086290912492315No Hit
ATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACC250400.12067014531451656No Hit
TAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGT249550.12026052221740259No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCCTGTAGGC249230.12010631116907731No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCTGTAGGCACCATCAA247610.11932561773693069No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA244360.11775941177737727No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT244340.11774977358685694No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATATCTCGTA243060.11713292939355589No Hit
CTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA241550.11640524600927107No Hit
CTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC237120.11427038680901823No Hit
TTGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC236470.11395714561710756No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTGTAGGCACCATCAATA236350.11389931647398559No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA232780.11217889946610689No Hit
CTTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGT229840.11076208545961856No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA225420.1086320453546259No Hit
CGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCATC222560.10725378411021887No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGCACC221370.10668031177425931No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC218840.10546108067343771No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCCTGTAGGC217520.10482496009909602No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT217360.10474785457493338No Hit
GACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT216700.10442979428776254No Hit
TGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCACC208830.10063716631801316No Hit
GGGGCTGATTCTGGATTCGACGGGCTGTAGGCACCATCAATATCTCGTAT208660.10055524169859036No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)