FastQCFastQC Report
Sat 16 Sept 2023
SRR15446802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16068530
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4559732.83767712416755Illumina PCR Primer Index 6 (96% over 29bp)
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA1425240.886975971044022No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC1168320.7270858006301758No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT877720.5462354054789081No Hit
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG868890.5407401921644357No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT853800.5313491651072002No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC813470.5062504161861726No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT786470.48944738566626816No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA740650.4609320205395266No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA703910.43806745234318256No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT693780.4317632042258999TruSeq Adapter, Index 6 (96% over 27bp)
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAT639990.39828783342346813No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT615470.3830281923735401No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC603950.3758588993517142No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT588560.3662811719553687No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC560180.348619319875558No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA536870.33411270352670713No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT515040.3205271421841326No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT470270.29266522824427627No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT448880.27935349406572973No Hit
ACCCCGTGAACCTTTACTATAGCTTGACACTGAACACTGTAGGCACCATC419790.26124978451669195No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCTGTAGGCACCATCAA370410.23051890869917785No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA368220.22915599622367447No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC367360.22862078858489235No Hit
CGGTGATTGGCGCAGCCTGGTAGCGCACTTCGCTGTAGGCACCATCAATA351000.21843939675875768No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT339060.2110087232621777No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA335280.20865629898939106No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA329920.2053205862639582No Hit
GGGTCGTTAGCTCAGTTGGTAGAGCCTGTAGGCACCATCAATATCTCGTA321810.20027345376334985No Hit
CGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCATC312940.1947533470703294No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA312400.1944172864599313No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC309380.1925378363795568No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCAT296170.18431679811407764No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT293060.18238133793197014No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAT288570.1795870561899564No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATAT287310.17880291476569418No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCCTGTAGGCACCATCAATA278910.17357530527061282No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTGTAGGCACCATCAATATCTCG274780.17100506393553114No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC263730.16412826811164433No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA261520.16275290894686695No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA260440.1620807877260708No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCA257550.1602822411259773No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA256180.15942964291070807No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA247700.1541522466585307No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCTGTAGGCACCATCAATATC243690.15165668545909303No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC240280.14953452493787545No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT234660.14603700525188054No Hit
GGGTCGTTAGCTCAGTTGGTAGAGCTGTAGGCACCATCAATATCTCGTAT231660.14417000186078005No Hit
GTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCCTGTAGGCACCATCAAT224360.13962696027576885No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA219910.13685757191230313No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT216870.13496567514265462No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT216800.1349221117301956No Hit
TGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCACC211250.13146815545665969No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT205480.12787728560110972No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTGTAGGCACCATCAATA204080.1270060173519295No Hit
TGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA194670.12114985004851096No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT193150.12020390166368672No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT190700.11867918222762132No Hit
GGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATA189970.11822487806912019No Hit
ATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACC188950.11759009691614604No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATATCTCGTA187780.11686196559361683No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCCTGTAGGCAC180850.11254918776017471No Hit
CGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAATA176730.10998516976973004No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGTAGGCACCATCAATA170770.10627605636607705No Hit
TGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATA169850.1057035086594729No Hit
GGGGCTGATTCTGGATTCGACGGGCTGTAGGCACCATCAATATCTCGTAT167940.10451484983380557No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATAT167300.10411655577703748No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCCTGTAGGCACCATCAAT167080.10397964219502345No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGCTGTAGGCACCATCAATATCTC166750.1037742718220024No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC163620.10182636495062088RNA PCR Primer, Index 6 (96% over 26bp)
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT162480.10111690366200268No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)