FastQCFastQC Report
Sat 16 Sept 2023
SRR15446803_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446803_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19849853
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT2202601.1096303836607757No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC1834080.9239766158469788No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1497010.7541667940815481Illumina PCR Primer Index 6 (96% over 29bp)
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC1417510.7141161196508609No Hit
ATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACC1374910.6926550035408323No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA1290680.6502214399270363No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC1262550.636050050345461No Hit
TTGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC1185480.5972235663407685No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC1140110.5743669739015196No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA1137350.5729765353929825No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG1105960.557162816268715No Hit
AGGTAGCGCCTCGTGACTGTAGGCACCATCAATATCTCGTATGCCGTCTT1099810.5540645565486052Illumina PCR Primer Index 6 (95% over 22bp)
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT1066710.5373893700875266No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC1058030.5330165417345911No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT994070.5007946406454497No Hit
TTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCA949670.478426716812462No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT891440.4490914869747398No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT870370.4384767987954369No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA764530.3851565046854503No Hit
TAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCAC754040.3798718307888728No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT706390.355866615233876No Hit
TCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACCA701100.35320160809251333No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT685850.34551893155077773No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT681730.34344334942933835No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC675460.3402846358610313No Hit
AAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACC673280.33918639095211434No Hit
TGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCACC623560.31413834651571476No Hit
TTTGCCCCGAGATGAGTTCTCCCTGCTGTAGGCACCATCAATATCTCGTA571300.2878106956258064No Hit
TATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCAC560800.2825209839085458No Hit
AGATTCCCCCAGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT549430.2767929817918551Illumina PCR Primer Index 6 (95% over 21bp)
TTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATCT543850.2739818778506823No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCTGTAGGCACCATCAATATCTCG523610.26378532878807714No Hit
TTCCCCATGCGAGAGTAGGGAACTGCCAGGCACTGTAGGCACCATCAATA517130.26052082098542495No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC512580.258228612574612No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT501360.2525761777681679No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCTGTAGGCAC493100.24841493788392288No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT492320.24802198787064064No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCTGTAGGCACCATCAATATCT475930.23976499977103105No Hit
ATGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC448630.22601174930615356No Hit
TTGAGGCGAACCGGGGGAACTCTGTAGGCACCATCAATATCTCGTATGCC441850.22259610688300815No Hit
TTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTA431180.21722075221413478No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTATTCTGTAGGCACCAT430590.21692352079383156No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATA421810.21250031423406512No Hit
TAGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTA418450.21080760648454172No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT409180.20613754671130308No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCATC408940.20601663901490858No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT408430.20575971016007022No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATCTC407200.20514005821604828No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA387980.19545736686311985No Hit
AAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATA380690.1917847955851361No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT377730.1902936006629369No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT369030.18591069666863527No Hit
CTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC367890.18533638511076128No Hit
ACCCCGTGAACCTTTACTATAGCTTGACACTGAACACTGTAGGCACCATC366700.18473688444947176No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC353980.17832877654056178No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCACTGTAGGCACCATCAATAT350140.17639425339824935No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATAT347780.1752053277170365No Hit
ACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAA340060.1713161301496792No Hit
AACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCA339560.17106423911552393No Hit
GAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC336810.16967883842766998Illumina PCR Primer Index 6 (100% over 22bp)
ATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATC334820.168676312111732No Hit
GGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGC330250.1663740280595529No Hit
GGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT327550.16501381647511446TruSeq Adapter, Index 6 (95% over 22bp)
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC324430.1634420164219856No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC318310.16035887016392514No Hit
TAGGCTGAGGCGTGATGACGAGCTGTAGGCACCATCAATATCTCGTATGC303770.15303387889069003No Hit
CTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCA302140.15221271411934384No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC293840.14803132295236643No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA290230.14621266968576543No Hit
TAACCGTGAGGCTTAACCTGTAGGCACCATCAATATCTCGTATGCCGTCT289490.14583987095521564Illumina PCR Primer Index 6 (95% over 21bp)
TTTCGAAGGTTCGAATCCTTCCCCCACCACCACTGTAGGCACCATCAATA285960.14406152025407945No Hit
TGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCA281620.14187510607761175No Hit
ACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGCTGTAGGCACCA280730.14142674003681538No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTATCTGTAGGCACCATC279820.1409682983546528No Hit
TGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTGACTGTAGGCAC279570.14084235283757518No Hit
AAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC278230.14016728486603905TruSeq Adapter, Index 6 (95% over 23bp)
AAGGCTGAGGCGTGATGACGAGCTGTAGGCACCATCAATATCTCGTATGC264950.1334770589988752No Hit
TGTGCGGGTTCAAGTCCCGCTCCGGGTACCACTGTAGGCACCATCAATAT255540.1287364697360731No Hit
AAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT254520.12822261202639637No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCCTGTAGGCAC250040.12596566836036518No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA248880.12538128116112499No Hit
AAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCAC248630.12525533564404734No Hit
TTGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT247060.1244643977967998No Hit
TTGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATC246840.12435356574177149No Hit
ACCTGCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTC239800.12080693998086534No Hit
AGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTAT235930.1188573033765036No Hit
CTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT235150.11846435336322136No Hit
TAACCGTGAGGCTTAACCCTGTAGGCACCATCAATATCTCGTATGCCGTC234940.11835855912887616No Hit
GAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT234050.11791019308807979No Hit
TGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCT232110.11693285587555735No Hit
TGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCTGTAGGCACCA228320.11502352183666047No Hit
AGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCTGTAGGCACCAT225460.11358270512129233No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT223930.11281191855677722No Hit
ATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTGTAGGCACCA220320.11099326529017621No Hit
TTGAGGCGAACCGGGGGAACCTGTAGGCACCATCAATATCTCGTATGCCG217750.10969854537461815No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATAT215920.10877662418960986No Hit
TTCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACC215400.10851465751408838No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA215010.10831818250744728No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA213110.10736099657765727No Hit
AAGGCGCTTGAGAGAACTCGGGTGAAGGAACCTGTAGGCACCATCAATAT213000.10730558055014312No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA212730.10716955939169927No Hit
TTAGGTAGCGCCTCGTGACTGTAGGCACCATCAATATCTCGTATGCCGTC209220.10540128433192932No Hit
AGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCCTGTAGGCACC204900.10322494579682781No Hit
TTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCCTGTAGGCACC204860.1032047945140954No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA203840.10269093680441865No Hit
ATCTAAGTACCCCGAGGAAAAGAACTGTAGGCACCATCAATATCTCGTAT203750.10264559641827072No Hit
TTATCCTGTCTGAATATGGGGGGACTGTAGGCACCATCAATATCTCGTAT202880.10220730601884054No Hit
ATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGCTGTAGGCACCA201920.10172367523326242No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)