FastQCFastQC Report
Sat 16 Sept 2023
SRR15446804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23408019
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT4438631.896200613986173No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT4138111.7678172595468247No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC3084361.31765101523542No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT3036171.2970640531349533No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC2360041.0082185938075325No Hit
TTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGC2076460.8870720755993919No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1664720.7111750891863169Illumina PCR Primer Index 6 (96% over 29bp)
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT1456240.6221115934671789No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA1327200.5669851857177662No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1254690.5360086216608078No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG1129970.4827277353115614No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1038940.4438393526594454No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1020760.4360727834337455No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT906830.38740142854463677No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT864390.36927088960411386No Hit
TTGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC836930.35753986700027884No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA792490.3385549200041234No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT778520.33258687973552997No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATAT766210.32732799815311153No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC763540.3261873633988421No Hit
GATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC740470.3163317664771205No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG697960.29817132325465046No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA694520.2967017413989625No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC669360.28595328805910486No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGCACC649660.27753736871112417No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT641760.2741624568913756Illumina PCR Primer Index 6 (95% over 21bp)
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATAT637830.2724835450620576No Hit
CATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC618160.26408044183491136No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC582210.24872245703491613No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC576020.24607806410273333No Hit
AAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTGTAGGCA555750.23741863845889735No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC505510.2159559081014075No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC493090.21065003407592925No Hit
AAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA487310.20818079479515117No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATA484140.20682655802697356No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA484120.20681801394641727No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGCTGTAGGCAC476940.20375068902669635No Hit
ACCCCGTGAACCTTTACTATAGCTTGACACTGAACACTGTAGGCACCATC469330.20049966637501448No Hit
ATCACGGGAGACACACGGCGGGTGCTGTAGGCACCATCAATATCTCGTAT468260.20004255806525106No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT467140.19956408955409682No Hit
CTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGC466060.19910270920405526No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG452730.19340807951326425No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT451850.1930321399687859No Hit
TACTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC450730.1925536714576317No Hit
TTGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATATCTCGTATG447360.1911139938838908No Hit
GTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGC440400.1881406538502895No Hit
AGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCCTGTAGGCACC439350.18769208962108241No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA431360.18427872943883034No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA426510.1822067899039214No Hit
AGGTAGCGCCTCGTGACTGTAGGCACCATCAATATCTCGTATGCCGTCTT417810.17849011486191976Illumina PCR Primer Index 6 (95% over 22bp)
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC404210.17268014008361834No Hit
TTACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTGTAGGCACC400280.17100122825430036No Hit
TAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGT361510.15443852809586323No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA358000.15293904195822808No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA356730.1523964928429014No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCCTGTAGGC353250.15090982282610074No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA349770.1494231528093001No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA340790.1455868606395099No Hit
TGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCACC335930.1435106490643228No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA333690.1425537120420143No Hit
AATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC326050.13928987326949793No Hit
ATGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC320460.13690180275400496No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT318860.1362182763094989No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC316430.13518017052190534No Hit
TTAGGTAGCGCCTCGTGACTGTAGGCACCATCAATATCTCGTATGCCGTC310770.1327621957244652No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCTCTGTAGGCACCATCAATAT310500.1326468506369548No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT303550.12967778264363167No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATCTC298680.12759729902816638No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA295730.12633704714610836No Hit
ATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACC290980.124307828013981No Hit
GACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT286450.12237259376797328No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACCTGTAGGCACCAT281440.12023230158861371No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTGTAGGCACCATCAATATCTCGT280980.12003578773581822No Hit
GAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT275250.11758790865643094No Hit
TACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTC269240.1150204124492551No Hit
TTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCA267280.1141830925547352No Hit
TAGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTA267070.11409337970889379No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGCAC254920.10890285077092597No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATATCTC240360.10268276012592094No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA238650.10195224123835511No Hit
AAGGCTGAGGCGTGATGACGAGGCTGTAGGCACCATCAATATCTCGTATG238250.10178135962722862No Hit
CAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT237650.10152503721053882No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)