FastQCFastQC Report
Sat 16 Sept 2023
SRR15446805_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446805_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15460524
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3003711.942825482499817Illumina PCR Primer Index 6 (96% over 29bp)
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT2082541.3470047975088038No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC1494150.9664290809289516No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC1171550.7577686241423641No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC1001230.6476041821092222No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA879590.5689263830902497No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT850230.5499360823734047TruSeq Adapter, Index 6 (96% over 27bp)
AGGTAGCGCCTCGTGACTGTAGGCACCATCAATATCTCGTATGCCGTCTT756330.48920075412709163Illumina PCR Primer Index 6 (95% over 22bp)
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC714910.46240994160353166No Hit
TTGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC689820.44618151364080544No Hit
ATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACC688450.44529538584850037No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC670470.4336657670852553No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT596800.3860153769691118No Hit
TGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCACC548680.3548909467751546No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT516030.3337726457395623No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC515130.3331905179927925No Hit
ACCCCGTGAACCTTTACTATAGCTTGACACTGAACACTGTAGGCACCATC490200.31706557940726976No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATAT489060.3163282175946947No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT479300.3100153655852803No Hit
TTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATCT467100.30212430057351225No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC431400.2790332332849779No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT423290.27378761547797476No Hit
TTCCCCATGCGAGAGTAGGGAACTGCCAGGCACTGTAGGCACCATCAATA411620.26623935902819335No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCACTGTAGGCACCATCAATAT393050.25422812318651034No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT390080.2523071016221701No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC389730.2520807186095374No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA382190.24720378170882176No Hit
AGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCCTGTAGGCACC368250.23818726971996548No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT367710.23783799307190365No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCTGTAGGCACCATCAATATCT366780.2372364610669082No Hit
TTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCA365710.2365443758568597No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATCTC364730.23591050342148817No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT351670.22746318300725124No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT350870.2269457361212337No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA344740.222980799357124No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT338800.21913875622844348No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT316440.20467611576425226No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA306400.19818215734473166No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCTGTAGGCACCATCAATATCTCG305070.19732190189672744No Hit
ACTTCGAAGGTTCGAATCCTTCCCCCACCACCACTGTAGGCACCATCAAT285700.18479321916902688No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA273120.1766563668864005No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTATTCTGTAGGCACCAT266860.17260734500331296No Hit
TAGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTA264980.17139134482117166No Hit
AGATTCCCCCAGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT264950.171371940562946Illumina PCR Primer Index 6 (95% over 21bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGTAGGCACCATCAATA259320.1677304081025973No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA254210.16442521611816002No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT252220.1631380669891913No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA247940.16036972614899728No Hit
AAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT246570.15948359835669218No Hit
TCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACCA245850.15901789615927636No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCTGTAGGCAC244600.1582093853998739No Hit
AAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTGTAGGCA244540.15817057688342256No Hit
TTGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT232300.15025363952735366No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA232040.15008546928939798No Hit
ATGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC227880.14739474548210654No Hit
CTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT224930.14548666008991673No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATC223350.14446470249003204No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA215280.13924495702732972No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC214450.1387081058830865No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA211450.1367676800605206No Hit
AGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTAT210340.1360497225061712No Hit
AAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACC203740.1317807856965262No Hit
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG203680.13174197718007488No Hit
GAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC202540.13100461536749983Illumina PCR Primer Index 6 (100% over 22bp)
GCGGGAATAGCTCAGTTGGTAGAGCACGCTGTAGGCACCATCAATATCTC201260.1301767003498717No Hit
ACCCCGTGAACCTTTACTATAGCTTGACACTGAACCTGTAGGCACCATCA200420.12963338111955325No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT192330.1244006994847005No Hit
TTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCAC189340.12246674174820982No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATAT189200.12237618854315675No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCCTGTAGGCAC187950.12156767778375428No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC187560.12131542242682072RNA PCR Primer, Index 6 (96% over 26bp)
GGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT187240.12110844367241369TruSeq Adapter, Index 6 (95% over 22bp)
AGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCTGTAGGCACCAT183950.1189804433536664No Hit
AACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCA183790.11887695397646289No Hit
ACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGCTGTAGGCACCA181360.1173052090601845No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGCTGTAGGCACCATCAAT178840.11567525136922914No Hit
ACGATGTCGACTTGGAGGTTGTGCCTGTAGGCACCATCAATATCTCGTAT178310.1153324428072425No Hit
TCTAAGTACCCCGAGGAAAAGAACTGTAGGCACCATCAATATCTCGTATG178040.11515780448321156No Hit
ACTTGTGGCTGGGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT177940.11509312362245937TruSeq Adapter, Index 6 (95% over 24bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAT177300.11467916611364531No Hit
TTGAGGCGAACCGGGGGAACTCTGTAGGCACCATCAATATCTCGTATGCC175770.1136895489441367No Hit
CTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCA175300.11338554889860136No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG175100.11325618717709697No Hit
AGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCTGTAGGCACCA173070.11194316570382738No Hit
TGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCA169370.10954997385599607No Hit
ATCTAAGTACCCCGAGGAAAAGAACTGTAGGCACCATCAATATCTCGTAT167660.10844393113713352No Hit
ACTAACTGGAGGACCGAACCGACCTGTAGGCACCATCAATATCTCGTATG167230.10816580343589906No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA165230.10687218622085512No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA164540.10642588828166497No Hit
TTTGCCCCGAGATGAGTTCTCCCTGCTGTAGGCACCATCAATATCTCGTA163640.10584376053489519No Hit
TTGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATATCTCGTATG159330.1030560154364755No Hit
CTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC159190.10296546223142243No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCA158780.10270027070233842No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAACTGTAGGCACCATCAATATC158370.10243507917325441No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC156620.10130316411009098No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCATC156480.10121261090503789No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT156150.10099916406455564Illumina PCR Primer Index 6 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)