FastQCFastQC Report
Sat 16 Sept 2023
SRR15446806_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446806_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16677054
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA4346332.6061737282855835No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA2067801.2399072402116105No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT1828341.096320729068815No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1654640.9921656426848532Illumina PCR Primer Index 6 (96% over 29bp)
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA1564740.9382592393116914No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT1522240.9127751220329442No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC1264130.7580055806019457No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC1228890.7368747501806974No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC1133130.6794545367545132No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC1002700.6012452798917602No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT943440.565711426010853No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT923780.5539227731708489No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC789770.47356685419379224No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT759210.45524227480465074No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA754890.4526518892365522No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA703490.42183109798649093No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT702840.42144134089869834No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA683080.40959272542980313No Hit
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG654250.3923054995204789No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC634450.38043289900002725No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT612800.36745098984508895No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT557360.3342077083878244No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC550040.3298184439529907No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT497720.29844599651713066No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA423710.25406765487477584No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT414650.24863504069723585No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA414320.24843716402189497No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA406520.24376007896838373No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCA368510.22096828372685007No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA360550.21619525846711296No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA354980.2128553400378748No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC351010.21047482367089537No Hit
GGGGGCTGATTCTGGATTCGACGGGCTGTAGGCACCATCAATATCTCGTA321750.19292975845733906No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA316840.18998559337878262No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC312900.18762306580047053No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTCTGTAGGCACCATCAATATCT312800.18756310317157934No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC311850.18699345819711322No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGG309300.1854644111603884No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC309200.18540444853149723No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC305400.18312586863363278No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCAT295820.17738144878585868No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAT294510.17659593834738438No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT285200.17101341759761648No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA279380.16752359259615038No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA275890.16543089684784856No Hit
ACTGTTTCGGCAAGGGGGTCCTGTAGGCACCATCAATATCTCGTATGCCG270730.1623368251970642No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA269530.16161727365037015No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC268890.16123351282546666No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA260480.15619065573571927No Hit
TGGGGCTGATTCTGGATTCGACGGGCTGTAGGCACCATCAATATCTCGTA255700.15332444207472135No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA235430.1411700171984812No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA234580.14066033485290627No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT230350.1381239156508098No Hit
GGGTCGTTAGCTCAGTTGGTAGAGCCTGTAGGCACCATCAATATCTCGTA230310.13809993059925332No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT228770.1371765061143293No Hit
TGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCCTGTAGGCACCATCAA221560.13285320057127595No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA221360.13273327531349363No Hit
CGGTGATTGGCGCAGCCTGGTAGCGCACTTCGCTGTAGGCACCATCAATA218000.1307185309827503No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA211900.12706081062038896No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA203940.12228778536065182No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC202660.12152026371084486No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT201440.12078871963837258No Hit
TGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATA196030.1175447414153603No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC192750.11557796718772992No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC189510.11363517801165601No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC189190.11344329759920427No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTGTAGGCACCATCAATATCTCG188060.11276571989273405No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC186790.1120041945058162No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT185240.11107477375800308No Hit
CGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA183980.11031924463397431No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA180460.10820856009700515No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATCTC179560.1076688964369846No Hit
GGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATA179400.10757295623075876No Hit
CGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT179210.10745902723586552No Hit
AAGGCTGAGGCGTGATGACCTGTAGGCACCATCAATATCTCGTATGCCGT178210.10685940094695381No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC174000.10433497427063557No Hit
TGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATATCT170360.10215233457899699No Hit
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTCG167940.10070123895983069No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)