FastQCFastQC Report
Sat 16 Sept 2023
SRR15446808_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446808_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12832382
Total Bases962.4 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA7225805.630910925189104Illumina PCR Primer Index 6 (96% over 29bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC1892221.474566452276748No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT1420241.106762563645627TruSeq Adapter, Index 6 (96% over 27bp)
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG1374141.070837822627163No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1250700.9746436787807594No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA1125390.8769922840513944No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC1110680.8655290966244615No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC880200.6859209771030819No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT825320.6431541704416218No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA753350.5870694934112779No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC731510.570050049943962No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT712010.5548541182767158No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT689580.5373749004666476No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC644060.5019021410054657No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT643030.5010994841020163No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT578620.45090615288728153No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT550650.4291097319266212No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA501190.39056661499010864No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC493940.3849168455240812No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA483410.37671104242376824No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCA480010.37406149536383815No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT439040.34213445329168035No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA426280.3321908590314721No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC404110.31491425364363373No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA402590.3137297502521356No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA396190.30874236755109064No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA386490.3011833656448195No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT383450.29881435886182317No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA375320.292478824274402No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG358750.27956617874997797No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT342220.26668470436743547No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT326540.25446561674987545No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA309190.24094513395876155No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATA306230.23863846945952824No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA304660.23741500214067815No Hit
TGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCA285630.22258533139054No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC281230.21915650578357157RNA PCR Primer, Index 6 (96% over 26bp)
CTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT279450.21776938996984346RNA PCR Primer, Index 6 (96% over 28bp)
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC273340.21300799804743967No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC271970.21194038643799723No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAT267680.20859728147120307No Hit
GTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCCTGTAGGCACCATCAAT259210.201996792177789No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGCTGTAGGCACCATCAAT258060.20110062184869495No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCTGTAGGCACCATCAA254860.19860693049817252No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA250450.19517031210573374No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTGTAGGCACCATCAATA243340.18962964163629167No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGCTGTAGGCACCATCAATATCTC242690.18912311058071682No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAT234180.18249145014542117No Hit
TGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT231830.18066014555988125Illumina PCR Primer Index 6 (100% over 23bp)
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC227710.17744951794608357No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT220540.17186209076381923No Hit
GATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT218810.170513938877443RNA PCR Primer, Index 6 (96% over 27bp)
GGGGCTGATTCTGGATTCGACGGGCTGTAGGCACCATCAATATCTCGTAT218510.17028015531333152No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT218030.16990610161075317No Hit
AGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATG214090.1668357441354224No Hit
GGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGC212810.16583826759521342No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCAT209670.16339133295751326No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCCTGTAGGCACCATC206150.16064827247193855No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCA203880.15887931017016169No Hit
AACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCA203520.15859876989322794No Hit
AAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACC198980.1550608452896742No Hit
GTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGA196960.1534867026246569Illumina PCR Primer Index 6 (100% over 28bp)
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA192860.15029166058180002No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA190460.14842139206890817No Hit
GCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG186780.14555364701580736TruSeq Adapter, Index 6 (96% over 25bp)
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC185460.14452499933371685No Hit
AGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT185080.1442288734858423RNA PCR Primer, Index 6 (95% over 24bp)
CGGTGATTGGCGCAGCCTGGTAGCGCACTTCGCTGTAGGCACCATCAATA184730.1439561259943789No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT183130.14270928031911767No Hit
CACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC182570.14227288433277624RNA PCR Primer, Index 6 (96% over 26bp)
TGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG180770.14087018294810738TruSeq Adapter, Index 6 (96% over 29bp)
ACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAA176840.137807618258247No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAA173020.13483077420856082No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGTAGGCACCATCAATA167860.13080969690584338No Hit
TGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC166890.13005379671521625TruSeq Adapter, Index 6 (96% over 26bp)
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT161450.12581452141932808No Hit
GCCCGGATAGCTCAGTCGGTAGAGCAGGGGCTGTAGGCACCATCAATATC160060.1247313242389449No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA157400.12265844330382308No Hit
AAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATA151990.11844254636434606No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT151990.11844254636434606TruSeq Adapter, Index 6 (95% over 22bp)
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA150580.11734376361302212No Hit
GTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTGTAGGCACCATCAATA148150.11545011674371913No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCTGTAGGCACCAT147730.11512281975396305No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCTGTAGGCACCATCAATATC145900.11369674001288302No Hit
CGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAATA142950.11139786829912014No Hit
GGGTCGTTAGCTCAGTTGGTAGAGCTGTAGGCACCATCAATATCTCGTAT142900.11135890437176824No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC142040.11068872482131534No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA140560.10953539257169868No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATATCTCGTA138330.10779760141180336No Hit
GTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG137750.10734561985452117RNA PCR Primer, Index 6 (96% over 25bp)
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC133400.10395575817490471No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCTGTAGGCACCATCA133070.10369859625438207No Hit
ACCTGCGGTTGGATCACCTGTAGGCACCATCAATATCTCGTATGCCGTCT130930.10203094016372019Illumina PCR Primer Index 6 (95% over 21bp)
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC130340.1015711658209676No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA128370.10003598708330223No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)