FastQCFastQC Report
Sat 16 Sept 2023
SRR15446810_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446810_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18632912
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3654311.9612125039822008Illumina PCR Primer Index 6 (96% over 29bp)
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA3273751.7569717497726605No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT2516551.350594045632803No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1763170.9462664772956584No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT1092320.5862315026228857No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA956970.5135912196655037No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC923870.4958269539404254No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC917960.4926551469786365No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC910790.4888071172128114No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC868390.46605168317222767No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC851420.45694414270834316No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT815360.4375912900785449No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT794490.4263906790307387No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT772540.41461044843661576No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA766120.41116493224462175No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG722850.387942582458394No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT717760.38521085700399377No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC711670.38194244678448547No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT701280.3763662920750122No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA663540.3561118090398323No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA656410.35228524666461153No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT614570.3298303560924884No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC604790.3245815790897311No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA593090.3183023673379663No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA591530.31746513910439766No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT586780.31491588647013413No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA580530.3115616066882085No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA570000.3059103161116201No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC565930.30372600911763015No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA544730.2923482920973383No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA513650.27566812959777837No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT506210.27167519494537407No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA505610.2713531840863092No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC504540.27077893138764353No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC504060.27052132270039164No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA476940.2559664318706598No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA456380.2449321931000372No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA430610.2311018267032013No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT430570.231080359312597No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA423620.22735040019509564No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT383430.2057810394854009No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA382860.20547512916928926No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC371240.19923885219873308No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT366720.19681303706044445No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA364350.19554109416713822No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATC325190.17452451876550482No Hit
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG309880.16630787501169972No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC299720.16085515779820136No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCAT297520.15967445131496355No Hit
AAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA295800.1587513535189776No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA293420.15747404377802032No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATCTC287760.15443640800750844No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT282560.15164564722894627No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC281470.15106066083497843No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAT280890.15074938367121574No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT279550.15003022608597089No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATA276110.148184030493999No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC275670.14794788919735144No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGCTGTAGGCACCATCAAT271190.14554354144966714No Hit
GCCCGGATAGCTCAGTCGGTAGAGCAGGGGCTGTAGGCACCATCAATATC263890.14162574266437794No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC259180.1390979574207188No Hit
TGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT258680.13882961503816474Illumina PCR Primer Index 6 (100% over 23bp)
CTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC255410.13707465585626122No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA254320.13648966946229338No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT253340.13596371839248744No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT248000.13309782174681017No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC247560.1328616804501626No Hit
GAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT235250.1262550909916818No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC234370.12578280839838668No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA231960.12448939811447615No Hit
CGGTGATTGGCGCAGCCTGGTAGCGCACTTCGCTGTAGGCACCATCAATA229220.12301888185807994No Hit
TGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCACC224710.12059843356744238No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT224550.1205125640050251No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC223900.12016371890770483No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC218490.11726025432846995No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT217130.11653036304792296No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA211940.11374496911701187No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT210240.1128326050163281No Hit
TGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATA207520.11137282245523406No Hit
ACGATGTCGACTTGGAGGTTGTGCCTGTAGGCACCATCAATATCTCGTAT204980.11000964315185946No Hit
ACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAA201360.10806684430216813No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGG200790.1077609339860565No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTGTAGGCACCATCAATATCTCG199840.10725108345920381No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCA199790.1072242492209484No Hit
ACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGCTGTAGGCACCA194080.10415977921218111No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT194030.10413294497392571No Hit
AGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCCTGTAGGCACCATCAA189990.10196473852288897No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)