FastQCFastQC Report
Sat 16 Sept 2023
SRR15446811_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446811_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15554007
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3483502.239615810896832Illumina PCR Primer Index 6 (96% over 29bp)
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA2170831.3956725106270043No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT1147490.7377455854301724No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG1120190.7201938381537311No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA1003320.6450556438607749No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT969040.6230163069876463TruSeq Adapter, Index 6 (96% over 27bp)
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC935900.6017099002205669No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT920740.5919632156524038No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC807580.519210258809836No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC766160.492580464956715No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC732420.4708883055022413No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC614710.39521005744693316No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT593850.3817987223485241No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT572400.36800806377417733No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA567140.3646262985480205No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC558380.35899430931206344No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC539650.3469523962539042No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT537620.3456472663282201No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT529210.34024029949324314No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT496180.31900461405218605No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT487230.31325046979855414No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC442760.2846597664511788No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA403850.2596437046736574No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC395070.25399885701478725No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA357900.23010147803070938No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC353580.22732405868147032No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC353560.2273112002585572RNA PCR Primer, Index 6 (96% over 26bp)
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC334430.21501211874213505No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC330930.2127618947323349No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA329320.21172679168782682No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT317610.20419818507218107No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC317200.20393458740246162No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA316690.20360669761817649No Hit
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG313850.20178080156451006No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATCTC308740.19849547451020175No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA308170.19812900945717715No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT307900.19795542074784972No Hit
GAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT304410.19571162594950614No Hit
TGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCT303990.1954415990683301No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA303310.1950044126892832No Hit
ACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAA298840.19213055516819558No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC296480.19061326126444458No Hit
ATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATC292660.1881573024880341No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA290150.18654357041243455No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA285160.18333539389560516No Hit
AACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCA283070.18199168870118165No Hit
AAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATA280050.1800500668412969No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA279820.17990219497779575No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA278560.1790921143342677No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA269860.17349870036705012No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA264950.17034195754187328No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA258860.166426567764821No Hit
ACCTGCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTC258410.16613725324927525No Hit
TGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT257760.16571935450459807Illumina PCR Primer Index 6 (100% over 23bp)
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT251260.16154036705782632No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA245410.15777927835573174No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT239660.15408248176820288No Hit
TTGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATC239310.15385745936722287No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT233820.15032782227756486No Hit
GGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT222660.1431528222920306TruSeq Adapter, Index 6 (95% over 22bp)
AAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC221970.14270920670152715TruSeq Adapter, Index 6 (95% over 23bp)
GCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATG216670.13930172462954402No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC213050.13697435008226497No Hit
ACCTGCGGTTGGATCACCTGTAGGCACCATCAATATCTCGTATGCCGTCT211650.13607426047834492Illumina PCR Primer Index 6 (95% over 21bp)
TGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTAT209340.13458911263187678No Hit
CTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT207390.13333541639784527RNA PCR Primer, Index 6 (96% over 28bp)
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAA205500.13212029543255316No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA202620.13026868253306045No Hit
TAACCGTGAGGCTTAACCCTGTAGGCACCATCAATATCTCGTATGCCGTC200950.12919500421981295No Hit
CCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGT200080.12863566282309116No Hit
ACGATGTCGACTTGGAGGTTGTGCCTGTAGGCACCATCAATATCTCGTAT199660.12836563594191516No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA195740.12584538505093895No Hit
AAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT182310.11721095406476287No Hit
CAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT179240.11523718614759527No Hit
TGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC177500.11411850335415177TruSeq Adapter, Index 6 (96% over 26bp)
AGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATC176210.11328913507625399No Hit
CTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC176050.11318626769294883No Hit
GAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC174340.11208687253387503Illumina PCR Primer Index 6 (100% over 22bp)
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT168550.10836435910051988No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT168050.10804289852769128No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGTAGGCACCATCAATA166770.10721995946125007No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC165540.10642916645209174No Hit
TGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCACC164010.10544549709923623No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGCTGTAGGCACCATCAAT162910.10473828383901333No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA161010.10351673366226465No Hit
TGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG160940.10347172918206864TruSeq Adapter, Index 6 (96% over 29bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT157450.10122793438372504No Hit
CTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA156330.10050786270058899No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCAT156050.10032784477980497No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)