FastQCFastQC Report
Thu 29 Jun 2023
SRR1548656.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548656.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99973197
Total Bases9.9 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATAGA1021064510.213382492909574No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT14062311.4066080131457634No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATAG12276651.2279941392691482No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG11221901.1224908612255342No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA10740701.0743579601640627No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA9412800.9415323589181608Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA8734390.8736731706199213No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTAGGCACCATCAATAG8173490.8175681327866308No Hit
TTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATAGATCG7923760.7925884374788975No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCCTGTAGGCACCATCAATA7693240.7695302571948359No Hit
TCCCATATTGTCTAGTGGTTAGGATATCCGGCTCTGTAGGCACCATCAAT6341100.634280006070027No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTCTGTAGGCACCATC6101240.6102875753788287No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGACTGTAGGCACCATCAATA6019820.60214339249349No Hit
GCGCAGTGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA4699590.4700849968817142No Hit
TGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATAGATCGG4192980.41941041457341816No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATA3389410.33903187071230706No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATAG3221250.3222113623114403No Hit
GTGTAATGGTTAGCACTCAGGACTCTGTAGGCACCATCAATAGATCGGAA2894390.2895165991340659No Hit
AAGTGGTTGAGCAGCTGCCATACTGCGATCCACTCTGTAGGCACCATCAA2683200.2683919370908985No Hit
TCCCATATTGTCTAGTGGTTAGGATACCCGGCTCTGTAGGCACCATCAAT2333130.2333755516491085No Hit
ACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCCTGTAGGCACCATCAATAG2271700.2272309046993866No Hit
ACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATAGATCGGAAGAGC2227380.222797716471946No Hit
GCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATAGAT2206110.22067014621929118No Hit
TTGCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATAG2176230.21768134513093546No Hit
GTTCAGTGGTAGAATGCTCGCCTGCTGTAGGCACCATCAATAGATCGGAA2172630.21732124861426608No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGCTGTAGGCACCATCAA2153350.21539273171388126No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTCTGTAGGCACCATCAAT2131480.2132051453751149No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCTGTAGGCACCATCAATAGA2115060.21156270515186185No Hit
ATTCCTGCGTAGTACGAGAGGAACCGCAGGTCTGTAGGCACCATCAATAG2070440.20709950888136547No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGTAGGCACCATCAATAG2050370.20509197080093378No Hit
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCCTGTAGGCACCATC2047630.20481789734102435No Hit
ATGCAGATCGTATGGTCTTGTACCGACGACTGTAGGCACCATCAATAGAT2045250.20457983353278178No Hit
ATGCAGATCGTATGGTCTTGTACCGACGACCTGTAGGCACCATCAATAGA2012380.20129195228197014No Hit
ATGCAGATACCATGAAAGGCGTTGGTTGCTTCTGTAGGCACCATCAATAG1907350.1907861364081415No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTCTGTAGGCACCATCA1883920.18844250824548503No Hit
ACGCGGGAGACCGGGGTTCAATTCCCCGTCGGGGAGCTGTAGGCACCATC1862930.18634294549968228No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTCTGTAGGCACCATCAAT1847900.1848395425425877No Hit
AACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCCTGTAGGCACCATCAATA1832800.18332913770878007No Hit
ACTCGGCTCATGGGTCGATGAAGAACGCAGCCTGTAGGCACCATCAATAG1808880.18093649640913254No Hit
TTCCTGCGTAGTACGAGAGGAACCGCAGGTACTGTAGGCACCATCAATAG1804680.18051638380635163No Hit
ATTCCTGCGTAGTACGAGAGGAACCGCAGGTACTGTAGGCACCATCAATA1799110.17995923447361595No Hit
TCGATAGTATAGTGGTTAGTATCCCCGCCTGTAGGCACCATCAATAGATC1747080.1747548395396418No Hit
ATATTGTCTAGTGGTTAGGATATCCGGCTCTGTAGGCACCATCAATAGAT1686240.1686692084079296No Hit
ACGCGGGAGACCGGGGTTCAATTCCCCGTCGGGGACTGTAGGCACCATCA1677530.1677979748912101No Hit
TCGATAGTATAGTGGTTAGTATCCCCGCCCTGTAGGCACCATCAATAGAT1675910.16763593145870886No Hit
ACCCTGATGGAAGACCGAAACAGTTCTGACGCTGTAGGCACCATCAATAG1664290.16647361992434834No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGCTGTAGGCACCATCAATAG1647090.16475315878915026No Hit
ATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCCTGTAGGCACCA1541160.15415731878615427No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTCTGTAGGCACCATCAATAGAT1457030.1457420632452116No Hit
TCGTTCTGGGTAAATAGAGTTTCTAGCATTCTGTAGGCACCATCAATAGA1454660.14550499970507094No Hit
ATGCAGATCGTATGGTCTTGTACCGACGACACTGTAGGCACCATCAATAG1441210.14415963910807014No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGTCGGGGAGCTGTAGGCACCATCA1378060.13784294604482838No Hit
GTTCAGTGGTAGAATGCTCGCCTCTGTAGGCACCATCAATAGATCGGAAG1357140.13575038517573865No Hit
ATGGTAGATGGGCTAGAAGAGCATGACATCTGTAGGCACCATCAATAGAT1343760.13441202645545086No Hit
CCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATAGA1341430.13417896398771764No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGTAGGCACCATCAAT1336600.13369583449451958No Hit
ACCTGCCGAAGCAACTAGCCCTTAAAATGGACTGTAGGCACCATCAATAG1306920.13072703876820102No Hit
TAACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCCTGTAGGCACCATCAAT1277550.12778925135304015No Hit
TATGGTAGATGGGCTAGAAGAGCATGACATCTGTAGGCACCATCAATAGA1262360.12626984410631584No Hit
GCGCAGTGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATAGATCGGAAG1256770.12571069423737644No Hit
GTTCAGTGGTAGAATGCTCGCCCTGTAGGCACCATCAATAGATCGGAAGA1201360.12016820868497383No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGCTGTAGGCACCATCAATA1195840.11961606069274748No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGTCGGGGACTGTAGGCACCATCAA1189920.11902390197644674No Hit
GGCCGTGATCGTCTAGTGGTTAGGACCCCACGTCTGTAGGCACCATCAAT1167160.11674729177661489No Hit
ACCCTGATGGAAGACCGAAACAGTTCTGACGTCTGTAGGCACCATCAATA1115310.1115609016684742No Hit
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCATCTGTAGGCACCA1102580.11028756037480725No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTACTGTAGGCACCAT1099630.10999248128475876No Hit
GTCTAGGGGTATGATTTCCGCTTCTGTAGGCACCATCAATAGATCGGAAG1094720.10950134964674582No Hit
ATGCAGATACCATGAAAGGCGTTGGTTGCTCTGTAGGCACCATCAATAGA1093430.10937231506160595No Hit
TGCTTGGACTACATGGTTGAGGGTTGTACTGTAGGCACCATCAATAGATC1085770.1086061096955817No Hit
ACTGCGATCCACTGAAGCTTATCCTTTGCTTGCTGTAGGCACCATCAATA1076650.10769386518668599No Hit
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCACTGTAGGCACCAT1063210.10634950485778702No Hit
ACTGCGATCCACTGAAGCTTATCCTTTGCTTGACTGTAGGCACCATCAAT1063020.10633049976385171No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAGA1060680.10609643702801663No Hit
TACTGCGATCCACTGAAGCTTATCCTTTGCTTGCTGTAGGCACCATCAAT1048260.10485410404550731No Hit
TAAGTGGTTGAGCAGCTGCCATACTGCGATCCACTCTGTAGGCACCATCA1046650.10469306088110797No Hit
ATGCAGATCGTATGGTCTTGTACCGACGACAGACTGTAGGCACCATCAAT1033650.10339271234869082No Hit
CTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATAGATC1029460.10297360001401176No Hit
TCCTGCGTAGTACGAGAGGAACCGCAGGTACGGACCCTGTAGGCACCATC1026410.10266851824294466No Hit
ATGCAGATCGTATGGTCTTGTACCGACGACAGCTGTAGGCACCATCAATA1024170.10244445818812817No Hit
AGTGGTTGAGCAGCTGCCATACTGCGATCCACTCTGTAGGCACCATCAAT1020300.1020573544327086No Hit
TTCCTGCGTAGTACGAGAGGAACCGCAGGTCTGTAGGCACCATCAATAGA1019050.10193232091997619No Hit
ATAGCCGTACTACCATGGAAGTCTTTCGCCTGTAGGCACCATCAATAGAT1009240.10095105791205217No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)