FastQCFastQC Report
Wed 28 Jun 2023
SRR1548657.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548657.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67230885
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA41416556.160345799404545Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATAGA32723514.867332922956465No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATAG6986961.0392485536967124No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG4181880.6220176932075191No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT3342180.4971197389414106No Hit
TCCCATATTGTCTAGTGGTTAGGATATCCGGCTCTGTAGGCACCATCAAT3265040.485645845655609No Hit
TTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATAGATCG2651880.3944437143732379No Hit
GCGCAGTGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA2622440.3900647745452109No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATAG2404370.35762878920900715No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA2391850.35576654985279454No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA2391760.3557531631481573No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCCTGTAGGCACCATCAATA2389590.3554303948252355No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTAGGCACCATCAATAG2281620.33937081149534176No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGACTGTAGGCACCATCAATA1774160.2638906211036788No Hit
TGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATAGATCGG1745870.2596827336126841No Hit
ACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCCTGTAGGCACCATCAATAG1618870.2407926059578124No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTCTGTAGGCACCATC1585490.2358276259489965No Hit
TCGTTCTGGGTAAATAGAGTTTCTAGCATTCTGTAGGCACCATCAATAGA1439930.21417686231558608No Hit
AAGTGGTTGAGCAGCTGCCATACTGCGATCCACTCTGTAGGCACCATCAA1432720.21310443853297484No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATA1420100.2112273250604986No Hit
ACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATAGATCGGAAGAGC1404200.20886234057457373No Hit
TATGGTAGATGGGCTAGAAGAGCATGACATCTGTAGGCACCATCAATAGA1353990.20139404679858072No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTCTGTAGGCACCATCAAT1310870.19498032786568256No Hit
ATGGTAGATGGGCTAGAAGAGCATGACATCTGTAGGCACCATCAATAGAT1250960.18606924481211276No Hit
ATGCAGATCGTATGGTCTTGTACCGACGACTGTAGGCACCATCAATAGAT1223640.1820056362488758No Hit
ATTCCTGCGTAGTACGAGAGGAACCGCAGGTCTGTAGGCACCATCAATAG1165180.17331022788112932No Hit
AACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCCTGTAGGCACCATCAATA1134100.16868735254637807No Hit
GCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATAGAT1109170.164979235361843No Hit
ATGCAGATCGTATGGTCTTGTACCGACGACCTGTAGGCACCATCAATAGA1088680.16193152893941526No Hit
GTTCAGTGGTAGAATGCTCGCCTGCTGTAGGCACCATCAATAGATCGGAA1063000.15811185588290264No Hit
TCGTTCTGGGTAAATAGAGTTTCTAGCATCTGTAGGCACCATCAATAGAT962980.14323476479597136No Hit
ATGCAGATACCATGAAAGGCGTTGGTTGCTTCTGTAGGCACCATCAATAG944590.1404994148150809No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGCTGTAGGCACCATCAATA940300.13986131522736908No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTCTGTAGGCACCATCAAT906070.13476990523031193No Hit
AGGTTGAAGTCAGGGGAAACCCTGATGGAAGACCGCTGTAGGCACCATCA898930.13370789332908528No Hit
TTCCTGCGTAGTACGAGAGGAACCGCAGGTCTGTAGGCACCATCAATAGA890750.13249119061871636No Hit
CCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATAGA853890.12700859136392448No Hit
ACCCTGATGGAAGACCGAAACAGTTCTGACGCTGTAGGCACCATCAATAG850330.12647907282493753No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTCTGTAGGCACCATCAATAG840090.12495596331953684No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGCTGTAGGCACCATCAATAG833880.1240322806995624No Hit
GTGTAATGGTTAGCACTCAGGACTCTGTAGGCACCATCAATAGATCGGAA831030.12360836838604757No Hit
ACTGCGATCCACTGAAGCTTATCCTTTGCTTGACTGTAGGCACCATCAAT822160.12228903427345927No Hit
TCCCATATTGTCTAGTGGTTAGGATACCCGGCTCTGTAGGCACCATCAAT796990.1185452192098914No Hit
TTGAGATAGTCGGGCTTGATTGGGAAACAATACTGTAGGCACCATCAATA790410.11756650235974135No Hit
AAGTGGTTGAGCAGCTGCCATACTGCGATCCACTGCTGTAGGCACCATCA786730.117019134881238No Hit
GTTCAGTGGTAGAATGCTCGCCTCTGTAGGCACCATCAATAGATCGGAAG776190.11545140302704628No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGCTGTAGGCACCATCAA770780.11464671333718127No Hit
ATGCAGATCGTATGGTCTTGTACCGACGACACTGTAGGCACCATCAATAG762800.11345975885933973No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA758030.11275026351356225Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTCAGTGGTAGAATGCTCGCCCTGTAGGCACCATCAATAGATCGGAAGA750680.1116570159681819No Hit
CTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATAGATC739030.10992418142346334No Hit
TATGGTAGATGGGCTAGAAGAGCATGACACTGTAGGCACCATCAATAGAT734420.10923848466370775No Hit
GTCTAGGGGTATGATTTCCGCTTCTGTAGGCACCATCAATAGATCGGAAG727600.10822406993452488No Hit
TAAGTGGTTGAGCAGCTGCCATACTGCGATCCACTCTGTAGGCACCATCA723280.10758150811193397No Hit
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCCTGTAGGCACCATC716330.10654775703160832No Hit
TTGAGATAGTCGGGCTTGATTGGGAAACAATCTGTAGGCACCATCAATAG705170.10488780565658179No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTCTGTAGGCACCATCA700960.10426160536188094No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGTAGGCACCATCAATAG681350.10134479116257356No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)