FastQCFastQC Report
Wed 28 Jun 2023
SRR1548658_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548658_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14092100
Total Bases718.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3239072.298500578338218Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCATATTGTCTAGTGGTTAGGATATCCGGCTCTGTAGGCACCATCAAT2031011.4412401274472932No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACTGTAGGCACCATCAATAGATC1304400.925624995564891No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCCTGTAGGCACCATCAATA1270130.9013064057166781No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATA1121800.79604885006493No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGTAGGCACCATCAAT1048080.7437358520021856No Hit
ATGTTAGTTACTTGTTCCCCGGATAGTCTGTAGGCACCATCAATAGATCG916190.6501444071501054No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGCTGTAGGCACCATCAA901860.639975589159884No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG854390.6062900490345654No Hit
CTGTTGTGTCGATATTTTCTCCTCGGACTGTAGGCACCATCAATAGATCG802120.5691983451721178No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTCTGTAGGCACCATCAAT777230.5515359669602118No Hit
TATGTTAGTTACTTGTTCCCCGGATAGTCTGTAGGCACCATCAATAGATC695990.4938866457092981No Hit
TTATGTTAGTTACTTGTTCCCCGGATAGTCTGTAGGCACCATCAATAGAT671090.47621717132293984No Hit
GTTCAGTGGTAGAATGCTCGCCTGCTGTAGGCACCATCAATAGATCGGAA587360.4168009026333903No Hit
TACTGTTGTGTCGATATTTTCTCCTCGGACTGTAGGCACCATCAATAGAT523380.37139957848723754No Hit
GTCTAGGGGTATGATTTCCGCTTCTGTAGGCACCATCAATAGATCGGAAG481130.3414182414260472No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATAG466710.3311855578657546No Hit
TTATGTTAGTTACTTGTTCCCCGGATAGCTGTAGGCACCATCAATAGATC433320.30749143136934876No Hit
TCCCATATTGTCTAGTGGTTAGGATACCCGGCTCTGTAGGCACCATCAAT424030.3008990853031131No Hit
GCGCAGTGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA419870.29794707673093435No Hit
TCGTTCTGGGTAAATAGAGTTTCTAGCATTCTGTAGGCACCATCAATAGA418770.2971664975411755No Hit
CTGTTGTGTCGATATTTTCTCCTCGGACCTGTAGGCACCATCAATAGATC395390.2805756416715749No Hit
TCGTTCTGGGTAAATAGAGTTTCTAGCATCTGTAGGCACCATCAATAGAT385200.2733446399046274No Hit
GTTCAGTGGTAGAATGCTCGCCTCTGTAGGCACCATCAATAGATCGGAAG372870.2645950568048765No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTCTGTAGGCACCATCA356660.2530921580176127No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGTAGGCACCATCAATAGAT340620.24170989419603894No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTAGGCACCATCAATAG332500.23594780054072848No Hit
GGCCGTGATCGTCTAGTGGTTAGGACCCCACGTCTGTAGGCACCATCAAT321390.2280639507241646No Hit
GTTCAGTGGTAGAATGCTCGCCCTGTAGGCACCATCAATAGATCGGAAGA317460.22527515416438998No Hit
TATGTTAGTTACTTGTTCCCCGGATAGCTGTAGGCACCATCAATAGATCG310560.22037879379226658No Hit
GCGCAGTGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATAGATCGGAAG293120.20800306554736342No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCCTGTAGGCACCATCAATAGA280120.19877803875930486No Hit
TCGTTCTGGGTAAATAGAGTTTCTAGCACTGTAGGCACCATCAATAGATC273890.1943571220754891No Hit
GCGCAGTGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAATAGATCGGAA267630.1899149168683163No Hit
CTGTTGTGTCGATATTTTCTCCTCGGACCCTGTAGGCACCATCAATAGAT264070.1873886787632787No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA260020.18451472811007585No Hit
GTTCAGTGGTAGAATGCTCGCCTGCCTGTAGGCACCATCAATAGATCGGA255160.1810659873262324No Hit
ATATTGTCTAGTGGTTAGGATATCCGGCTCTGTAGGCACCATCAATAGAT251710.17861780714017073No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCCTGTAGGCACCATCAA247650.17573676031251553No Hit
TTTATGTTAGTTACTTGTTCCCCGGATAGTCTGTAGGCACCATCAATAGA235300.1669729848638599No Hit
CCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATAGA232600.16505701776172466No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA222180.1576628039823731No Hit
GTTCTGGGTAAATAGAGTTTCTAGCATTCTGTAGGCACCATCAATAGATC215740.15309286763505794No Hit
TCCCATATTGTCTAGTGGTTAGGATATCCGGCTCCTGTAGGCACCATCAA199870.14183123877917417No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT199790.14177446938355534No Hit
TTATGTTAGTTACTTGTTCCCCGGATACTGTAGGCACCATCAATAGATCG199320.14144094918429473No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGCTGTAGGCACCATCAATAG182480.12949099140653275No Hit
GTTCTGGGTAAATAGAGTTTCTAGCATCTGTAGGCACCATCAATAGATCG180840.12832721879634687No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATAGA177300.125815173040214No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGACTGTAGGCACCATCAATA170100.12070592743452005No Hit
TCGTTCTGGGTAAATAGAGTTTCTAGCATTTCTGTAGGCACCATCAATAG168910.11986148267469007No Hit
TTACTGTTGTGTCGATATTTTCTCCTCGGACTGTAGGCACCATCAATAGA162120.11504318022154257No Hit
TCGATAGTATAGTGGTTAGTATCCCCGCCCTGTAGGCACCATCAATAGAT161180.1143761398230214No Hit
CATATTGTCTAGTGGTTAGGATATCCGGCTCTGTAGGCACCATCAATAGA157050.11144541977419972No Hit
ATGTGCTCGTTCTGGGTAAATAGAGTTTCTAGCACTGTAGGCACCATCAA155220.11014681984941918No Hit
ATGTGCTCGTTCTGGGTAAATAGAGTTTCTAGCATCTGTAGGCACCATCA153160.10868500791223451No Hit
TACTGTTGTGTCGATATTTTCTCCTCGGACCTGTAGGCACCATCAATAGA152990.10856437294654452No Hit
TTTATGTTAGTTACTTGTTCCCCGGATAGCTGTAGGCACCATCAATAGAT150350.10669098289112339No Hit
CGTTCTGGGTAAATAGAGTTTCTAGCATCTGTAGGCACCATCAATAGATC149820.10631488564514871No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTCTGTAGGCACCATC145650.1033557808985176No Hit
CGTTCTGGGTAAATAGAGTTTCTAGCATTCTGTAGGCACCATCAATAGAT145100.1029654913036382No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)