FastQCFastQC Report
Thu 29 Jun 2023
SRR1548659.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548659.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189631476
Total Bases9.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATTCGT105454085.561000854098715No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATTCG90859514.7913728204066715No Hit
TTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATTCGTAT21079151.1115849775909565No Hit
AACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCCTGTAGGCACCATCAATT20892451.1017395656404636No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA19506771.028667308374481No Hit
ACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCCTGTAGGCACCATCAATTC18255200.9626671892803281No Hit
TCGTTCTGGGTAAATAGAGTTTCTAGCATTCTGTAGGCACCATCAATTCG17782840.9377578224408273No Hit
ATCCAGCTGGATAACGCAGAAGATATAGTCACTGTAGGCACCATCAATTC16728550.8821610395523156No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATTC15546550.819829615205864No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT12161030.6412980722672854No Hit
ACTGCGATCCACTGAAGCTTATCCTTTGCTTGACTGTAGGCACCATCAAT11637000.613663946801743No Hit
ATCCAGCTGGATAACGCAGAAGATATAGTCCTGTAGGCACCATCAATTCG11578780.6105937813825801No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGACTGTAGGCACCATCAATT10732380.5659598409707047No Hit
TCGTTCTGGGTAAATAGAGTTTCTAGCACTGTAGGCACCATCAATTCGTA9804590.5170338915676637No Hit
ATCCAGCTGGATAACGCAGAAGATATAGTCGCTGTAGGCACCATCAATTC9735740.5134031652002751No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA9582590.5053269743046245No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA9516700.5018523401674098No Hit
ATGTTAGTTACTTGTTCCCCGGATAGTCTGTAGGCACCATCAATTCGTAT9333310.49218147729863154No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATTC9037790.4765975665347877No Hit
ATCTGGCAGTGTAAGGAGACATAAGAGGCTGTAGGCACCATCAATTCGTA9034880.4764441109976912No Hit
TCGTTCTGGGTAAATAGAGTTTCTAGCATCTGTAGGCACCATCAATTCGT8494800.44796360705434785No Hit
AGGCAAGGTGTTGTAAGTGGTTGAGCAGCCTGTAGGCACCATCAATTCGT7921400.4177260108443178No Hit
AATCTGGCAGTGTAAGGAGACATAAGAGGCTGTAGGCACCATCAATTCGT7202470.3798140557636117No Hit
TCTGGCAGTGTAAGGAGACATAAGAGGCTGTAGGCACCATCAATTCGTAT6747290.3558106566654578No Hit
GTTCTGGGTAAATAGAGTTTCTAGCATTCTGTAGGCACCATCAATTCGTA6483230.3418857531858266No Hit
ACTGCGATCCACTGAAGCTTATCCTTTGCTTGCTGTAGGCACCATCAATT6468220.3410942179240328No Hit
ACTCTTATTGTTTCCTTACTTACTTGATTCTGTAGGCACCATCAATTCGT6196690.32677539249865883No Hit
TAATCTGGCAGTGTAAGGAGACATAAGAGGCTGTAGGCACCATCAATTCG6121750.32282351691445993No Hit
AACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGTAGGCACCATCAATTC5504350.29026563079644013No Hit
ATGAAAGTATCAACGTGTATTTCCTAGACCCTGTAGGCACCATCAATTCG5373990.28339124460540505No Hit
TAACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCCTGTAGGCACCATCAAT5305660.2797879398460201No Hit
TCGTTCTGGGTAAATAGAGTTTCTAGCATTTCTGTAGGCACCATCAATTC5035060.26551815691188313No Hit
ACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGTAGGCACCATCAATTCG4932020.2600844598182635No Hit
ACTGCGATCCACTGAAGCTTATCCTTTGCTCTGTAGGCACCATCAATTCG4632450.24428697691516152No Hit
ATGAAAGTATCAACGTGTATTTCCTAGACCGCTGTAGGCACCATCAATTC4538440.23932946659129523No Hit
AACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCTCTGTAGGCACCATCAAT4388950.23144628162890005No Hit
TGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATTCGTATG4300190.22676562407814618No Hit
ACTCTTATTGTTTCCTTACTTACTTGACTGTAGGCACCATCAATTCGTAT4035360.21280011552512515No Hit
ACCCTGATGGAAGACCGAAACAGTTCTGACGCTGTAGGCACCATCAATTC3933350.2074207343088971No Hit
ACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCTCTGTAGGCACCATCAATT3856910.2033897579323804No Hit
ACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCAATTCGTATG3771450.19888312212472575No Hit
GTTACTTGGATAACTGTGGTAATTCTAGACTGTAGGCACCATCAATTCGT3632610.1915615527877872No Hit
TTGGGGGCATTAGTATTACGACGCGAGAGGCTGTAGGCACCATCAATTCG3628370.19133796121483546No Hit
AGATACCATGAAAGGCGTTGGTTGCTTACTGTAGGCACCATCAATTCGTA3580280.18880198981312574No Hit
CCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATTCG3415280.1801009026581642No Hit
TCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATTCGT3155280.16639009865640658No Hit
AATCTGGCAGTGTAAGGAGACATAAGAGGTCTGTAGGCACCATCAATTCG3134190.16527794151641786No Hit
CTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATTCGTA3048890.16077974312661047No Hit
ACTACTCTTATTGTTTCCTTACTTACTTGACTGTAGGCACCATCAATTCG3013520.158914546443756No Hit
ACTCTTATTGTTTCCTTACTTACTTGATCTGTAGGCACCATCAATTCGTA2902090.15303841225177195No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCTGTAGGCACCATCAATTC2798030.1475509266193762No Hit
ACACACAGGGACAGCAAAAGCTCGGCCTATCCTGTAGGCACCATCAATTC2791190.14719022700640688No Hit
TTATGTTAGTTACTTGTTCCCCGGATAGTCTGTAGGCACCATCAATTCGT2748370.1449321630550405No Hit
AGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGTAGGCACCATCAATTCGTA2671830.14089591329236925No Hit
ATAGCCGTACTACCATGGAAGTCTTTCGCCTGTAGGCACCATCAATTCGT2648560.13966879633421195No Hit
AGTTACTTGGATAACTGTGGTAATTCTAGACTGTAGGCACCATCAATTCG2549670.134453944766005No Hit
GTTACTTGGATAACTGTGGTAATTCTAGAGCTGTAGGCACCATCAATTCG2548190.13437589865091806No Hit
TTCTGGGTAAATAGAGTTTCTAGCATTTCTGTAGGCACCATCAATTCGTA2539620.1339239694574755No Hit
AGGCAAGGTGTTGTAAGTGGTTGAGCAGCTCTGTAGGCACCATCAATTCG2502710.13197756262784138No Hit
TCTGGCAGTGTAAGGAGACATAAGAGGTCTGTAGGCACCATCAATTCGTA2437430.12853509614616931No Hit
TTGGGGGCATTAGTATTACGACGCGAGAGGTCTGTAGGCACCATCAATTC2420380.1276359838068233No Hit
TTGCCAAAGATGTTTTCATTAATCAAGACTGTAGGCACCATCAATTCGTA2344650.12364244847200367No Hit
GTTCTGGGTAAATAGAGTTTCTAGCATCTGTAGGCACCATCAATTCGTAT2341380.12347000874475078No Hit
ATGCAGATACCATGAAAGGCGTTGGTTGCTCTGTAGGCACCATCAATTCG2304870.12154469545973476No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGCTGTAGGCACCATCAATTC2286310.12056595498945544No Hit
ACAACCTCAACTCATATGGGACTACCCCCTCTGTAGGCACCATCAATTCG2281660.12032074253327016No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACTGTAGGCACCATCAATTCGTA2256280.11898235712725243No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTCTGTAGGCACCATCAATTC2248210.11855679486458251No Hit
ATACTGCGATCCACTGAAGCTTATCCTTTGCTTGCTGTAGGCACCATCAA2211550.11662357150033467No Hit
TAGCTAATTAACACAATCCCGGGGCGTTCTCTGTAGGCACCATCAATTCG2166720.11425951248726239No Hit
CAGGCAAGGTGTTGTAAGTGGTTGAGCAGCCTGTAGGCACCATCAATTCG2153280.11355076938809462No Hit
ATGCAGATACCATGAAAGGCGTTGGTTGCTTACTGTAGGCACCATCAATT2111570.11135124002304345No Hit
TACTGCGATCCACTGAAGCTTATCCTTTGCTTGACTGTAGGCACCATCAA2062440.1087604254053267No Hit
AGATACCATGAAAGGCGTTGGTTGCTTCTGTAGGCACCATCAATTCGTAT1981560.10449531068354918No Hit
ATACCACGCTGAATACATCGGTTCTCGTCCTGTAGGCACCATCAATTCGT1974670.10413197437750261No Hit
TTCTGGGTAAATAGAGTTTCTAGCATTCTGTAGGCACCATCAATTCGTAT1952200.10294704450858147No Hit
AATCCAGCTGGATAACGCAGAAGATATAGTCCTGTAGGCACCATCAATTC1942870.10245503758036455No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)