FastQCFastQC Report
Wed 28 Jun 2023
SRR1548661.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548661.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8689975
Total Bases868.9 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA357691541.161395746247834Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA650100.7481034180190391Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT311120.3580217434457521Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC281560.32400553511373736Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA181180.20849311994568456Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC142520.16400507481321866Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT136370.15692795433818854TruSeq Adapter, Index 4 (100% over 43bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAGT124240.14296934110857626No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACAGGTCTGAACTCCAGTCA98980.11390136335259883Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCAGTCAC98460.11330297267828733Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGACTCCAGTCAC97400.1120831763037293Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)