FastQCFastQC Report
Sat 16 Sept 2023
SRR15513149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145676168
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN106588627.316819316664068No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN94652776.49747802262344No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67265534.6174697566179805No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN23882491.6394232720344484No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN17913561.2296836363790131No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN16570721.1375038365918575No Hit
AACCTGCATTAAAAATTTCGGTTGGGNNNNNNNNN15955661.095282791897711No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN12483680.8569473079495062No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN9547410.6553858555642402No Hit
AAGCTTGGGCTGCAGGTCGACCCGNNNNNNNNNNN6036770.41439654013963356No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN5879980.40363362660665264No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN5776280.39651509778867877No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN4691810.32207121208734707No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN4562450.3131912420980211No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN4382870.3008638997148799No Hit
GCATGCTCATAAGGAAAGGTTAAAANNNNNNNNNN4209110.2889360736067687No Hit
CCCCTTGTAAATTTAACTGTTAGTCCANNNNNNNN3928460.269670739828906No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN3430450.23548464015061132No Hit
GCATGCTCATAAGGAAAGGTTAAAAANNNNNNNNN3362220.23080096395726168No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAANNNNNNN3314290.22751078954795131No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAANNNNNNN3146730.2160085649699407No Hit
GAGAGAGACGGGGAGGGCGGCGCCGCCGCNNNNNN3130480.2148930770886285No Hit
AAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACNNN3108430.213379445840448No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN3049660.20934515520754224No Hit
AGTTCAACTTTAAATTTGCCCACAGAACNNNNNNN2834380.19456717175591823No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN2785820.19123375073951698No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTNNNNNN2746780.18855383400804446No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN2712740.18621714431697572No Hit
TCCCCTTGTAAATTTAACTGTTAGTCCANNNNNNN2471960.16968870295929256No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN2386000.1637879436806712No Hit
AGCTGCGAGAATTAATGTGAATTGCAGNNNNNNNN2380060.16338018995667156No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAANNNNNNN2292490.15736891157104022No Hit
CCATTCGTGATGGGGATCGGGGATTNNNNNNNNNN2178870.14956942030490533No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN2144660.14722106089446285No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN2119900.14552140059038346No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN2027250.1391614035317019No Hit
GCTTGAGAGAACTCGGGTGAAGGAACNNNNNNNNN2002820.13748439621228917No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN1939710.13315218450831298No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN1899560.13039607137387083No Hit
AAGCTTGGGCTGCAGGTCGACCCNNNNNNNNNNNN1820570.12497377058957235No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN1783830.12245173829668556No Hit
GAAGGTGTGCTGTGAGGCATGCTGGAGGTNNNNNN1701100.11677270368616506No Hit
ATAAAGTATAGCGATAGAAATTGAAACNNNNNNNN1691930.11614322529406457No Hit
TTAACCAGTGAAATTGACCTGCCCGTGANNNNNNN1686630.11577940463123661No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTANNNNN1654930.11360334519507678No Hit
CGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNNN1634560.11220503823247191No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN1627900.11174785981465411No Hit
ATAAAGTATAGGCGATAGAAATTGAAACNNNNNNN1594700.10946883226637318No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGANNNNNNNN1587290.10896016979249483No Hit
ACCTATTCTCAAACTTTAAATGGGTAAGAANNNNN1564060.10736553696277897No Hit
TAGCTGCGAGAATTAATGTGAATTGCNNNNNNNNN1556970.10687884101948646No Hit
AGGAAAGGTTAAAAAAAGTAAAAGGAANNNNNNNN1554430.10670448168296136No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN1546430.10615531841831534No Hit
TTCGTGATGGGGATCGGGGATTNNNNNNNNNNNNN1495080.10263037671336879No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCNNN1479650.10157117806668281No Hit

[OK]Adapter Content

Adapter graph