FastQCFastQC Report
Sat 16 Sept 2023
SRR15513150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89615744
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN64506117.198077828824363No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN56143346.2648969359669655No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42153844.703843110424883No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN14895781.6621833770637444No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN11556631.2895758584563No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN10046281.1210396244659866No Hit
AACCTGCATTAAAAATTTCGGTTGGGNNNNNNNNN9567091.0675679934097295No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN7436160.8297827667424154No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN6697880.7473999211567111No Hit
AAGCTTGGGCTGCAGGTCGACCCGNNNNNNNNNNN3903790.4356143045579134No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN3699450.4128125075879524No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN3554940.3966869928569694No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN3072240.34282368955169307No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN2848340.31783924039061706No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN2815830.3142115296169387No Hit
CCCCTTGTAAATTTAACTGTTAGTCCANNNNNNNN2578140.2876882883436196No Hit
GCATGCTCATAAGGAAAGGTTAAAANNNNNNNNNN2565280.2862532726392363No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN2560530.28572323184640414No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN2217130.24740407221302543No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2121110.23668943707034337No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN2075970.23165237572540823No Hit
GCATGCTCATAAGGAAAGGTTAAAAANNNNNNNNN2046640.2283795133140891No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAANNNNNNN2039380.22756938780757094No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAANNNNNNN2000610.22324313906270754No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN1988530.22189516163588396No Hit
GAGAGAGACGGGGAGGGCGGCGCCGCCGCNNNNNN1914110.21359081725639636No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTNNNNNN1861960.2077715272887764No Hit
AAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACNNN1855160.20701273204851148No Hit
AGTTCAACTTTAAATTTGCCCACAGAACNNNNNNN1816730.20272442306566132No Hit
TCCCCTTGTAAATTTAACTGTTAGTCCANNNNNNN1691530.18875366364196006No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN1506890.16815014111806068No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAANNNNNNN1478570.16498998211742794No Hit
AGCTGCGAGAATTAATGTGAATTGCAGNNNNNNNN1453320.16217239685026774No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN1404900.15676932838944016No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN1322190.14753992334204133No Hit
CCATTCGTGATGGGGATCGGGGATTNNNNNNNNNN1302580.14535169177415969No Hit
GCTTGAGAGAACTCGGGTGAAGGAACNNNNNNNNN1248950.13936725225424676No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN1228870.13712657454475857No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN1171710.13074823102511987No Hit
AAGCTTGGGCTGCAGGTCGACCCNNNNNNNNNNNN1168920.13043690180154058No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN1107180.1235474873700764No Hit
CGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNNN1096840.12239367225473237No Hit
ATAAAGTATAGCGATAGAAATTGAAACNNNNNNNN1082780.12082475150794932No Hit
TTAACCAGTGAAATTGACCTGCCCGTGANNNNNNN1067610.11913196859694654No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN1048760.11702854355591803No Hit
ATAAAGTATAGGCGATAGAAATTGAAACNNNNNNN1036200.11562700411213457No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTANNNNN1033440.11531902251461529No Hit
GAAGGTGTGCTGTGAGGCATGCTGGAGGTNNNNNN1029200.11484589136480305No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGANNNNNNNN1027200.11462271629413688No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNNN985740.1099962970792275No Hit
ACCTATTCTCAAACTTTAAATGGGTAAGAANNNNN984490.10985681266006116No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCNNN983330.1097273711190748No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN980570.1094193895215555No Hit
TAGCTGCGAGAATTAATGTGAATTGCNNNNNNNNN964540.10763064133016625No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACNNN960950.10723004207832051No Hit
AGGAAAGGTTAAAAAAAGTAAAAGGAANNNNNNNN951870.10621682725749618No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNN944350.10537768899179145No Hit
TTCGTGATGGGGATCGGGGATTNNNNNNNNNNNNN938890.10476842104887284No Hit
AAGCCAGCTTGACTGCGAGCGTGACGGCGCNNNNN938000.1046691081424264No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACNNNN905470.10103916561804141No Hit
ATGCTCATAAGGAAAGGTTAAAANNNNNNNNNNNN899570.10038079915957625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)