FastQCFastQC Report
Sat 16 Sept 2023
SRR15513151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140962476
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN1513063610.733804079888607No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN82908435.8815957517658815No Hit
AACCTGCATTAAAAATTTCGGTTGGGNNNNNNNNN46573363.303954450970342No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN43759013.1043020271579227No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36162742.5654160615056165No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN14572261.0337687314743251No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN13115870.9304511648901513No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN12277370.8709672494685748No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN9570900.6789679261876757No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN9495780.6736388483982078No Hit
GAGAGAGACGGGGAGGGCGGCGCCGCCGCNNNNNN8180530.5803338755201775No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN6471480.4590923899492231No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN6145720.4359826937205615No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAANNNNNNN5962280.422969301418911No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN5575830.3955542040847807No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN5201100.36897053369011484No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNN5198240.36876764281580865No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN4498290.3191125842596579No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN3699440.26244147414096214No Hit
CCCCTTGTAAATTTAACTGTTAGTCCANNNNNNNN3690710.26182216038827244No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN3369530.2390373733219612No Hit
TTAACCAGTGAAATTGACCTGCCCGTGANNNNNNN3328800.23614795188472712No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACNNN3311710.23493557249945016No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN3301980.23424531788161837No Hit
AAGCTTGGGCTGCAGGTCGACCCGNNNNNNNNNNN3230830.22919787532676428No Hit
GCATGCTCATAAGGAAAGGTTAAAANNNNNNNNNN3211460.2278237507689635No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACNNNN3143930.22303311414592347No Hit
AAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACNNN3113380.22086587071583505No Hit
GCATGCTCATAAGGAAAGGTTAAAAANNNNNNNNN2770430.19653670101538226No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN2548280.18077718782408447No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN2529250.179427183160432No Hit
GAAGGTGTGCTGTGAGGCATGCTGGAGGTNNNNNN2411540.17107673392456585No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN2407370.17078091051692365No Hit
TCCCCTTGTAAATTTAACTGTTAGTCCANNNNNNN2388530.16944438461764816No Hit
ACCTATTCTCAAACTTTAAATGGGTAAGAAGNNNN2290770.16250920564136517No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNN2203070.15628769176841076No Hit
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAANNNNN2085930.14797767882567556No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTNNNNNN2035370.1443909086840955No Hit
TAACCAGTGAAATTGACCTGCCCGTGANNNNNNNN1973140.1399762586463081No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN1972000.13989538606004623No Hit
TTAACCAGTGAAATTGACCTGCCCGTGNNNNNNNN1903250.13501820158153294No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACNNNNN1902430.13496003007211652No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN1900120.13479615667363845No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGNNNNN1845850.1309461959223815No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN1845270.13090505022059915No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGNNNNNNN1816080.1288342863671038No Hit
ACCTGCATTAAAAATTTCGGTTGGGNNNNNNNNNN1806020.1281206212637752No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGANNN1805920.128113527177261No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN1780910.1263392961400593No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCNNNNNNN1764750.12519289175936438No Hit
AGACCCGGCGGCGTCCGCCCTCCTCTTGCCGCNNN1744150.12373150993743895No Hit
AAGGCGCTTGAGAGAACTCGGGTGAAGGAACNNNN1737820.12328245426109002No Hit
GAATACAAGCTTGGGCTGCAGGTCGACNNNNNNNN1680980.1192501754864181No Hit
GCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNNN1676130.11890611229047934No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAANNNNNNN1645600.11674028767769375No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAANNNNNNN1641770.11646858416419985No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCNNN1628010.11549243785984575No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACANNNNN1611020.114287152561083No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN1554700.11029176303628493No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN1513510.10736970880108547No Hit
GAGAGAGAGACGGGGAGGGCGGCGCCGCCGCNNNN1487250.10550680168245626No Hit
ATAAAGTATAGGCGATAGAAATTGAAACNNNNNNN1487200.10550325463919916No Hit
AGTTCAACTTTAAATTTGCCCACAGAACNNNNNNN1447110.10265923535565592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)