FastQCFastQC Report
Sat 16 Sept 2023
SRR15513152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76499364
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN796795110.4157088155661No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN43135655.638693937377048No Hit
AACCTGCATTAAAAATTTCGGTTGGGNNNNNNNNN25975873.395566791901695No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN24371723.185872238101221No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20026492.617863594264653No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN7705291.007235824862544No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN7203360.941623514673926No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN6407050.8375298388101632No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN4862480.6356235850535961No Hit
GAGAGAGACGGGGAGGGCGGCGCCGCCGCNNNNNN4557380.5957409005387286No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN4295350.5614883281905455No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN3419820.44703901067726526No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAANNNNNNN3355570.4386402480418007No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN3305950.4321539196064428No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN3044230.3979418704709754No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNN2975700.38898362605994996No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN2691010.35176893758227845No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN2688280.3514120718702969No Hit
CCCCTTGTAAATTTAACTGTTAGTCCANNNNNNNN2058420.2690767468341306No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN2011920.26299826492675155No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACNNN1868160.244205951829874No Hit
AAGCTTGGGCTGCAGGTCGACCCGNNNNNNNNNNN1846370.24135756213607215No Hit
TTAACCAGTGAAATTGACCTGCCCGTGANNNNNNN1839560.2404673586567334No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACNNNN1809320.23651438461632177No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN1767960.23110780371977993No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN1736640.2270136520350679No Hit
GCATGCTCATAAGGAAAGGTTAAAANNNNNNNNNN1728180.22590776048804798No Hit
AAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACNNN1712480.2238554558440512No Hit
GCATGCTCATAAGGAAAGGTTAAAAANNNNNNNNN1479490.19339899348705697No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN1465460.19156499131156177No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN1411410.18449957309449005No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN1371620.1792982226623479No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNN1333580.1743256323019888No Hit
GAAGGTGTGCTGTGAGGCATGCTGGAGGTNNNNNN1332210.17414654584579292No Hit
TCCCCTTGTAAATTTAACTGTTAGTCCANNNNNNN1321620.17276222061140273No Hit
ACCTATTCTCAAACTTTAAATGGGTAAGAAGNNNN1242670.16244187337296032No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTNNNNNN1165430.15234505740466026No Hit
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAANNNNN1128410.14750580148614045No Hit
TAACCAGTGAAATTGACCTGCCCGTGANNNNNNNN1102780.14415544683482598No Hit
TTAACCAGTGAAATTGACCTGCCCGTGNNNNNNNN1051610.1374665023358887No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACNNNNN1049340.1371697678427758No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGNNNNN1025870.13410176848006214No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCNNNNNNN1015230.132710907243621No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGNNNNNNN1008800.13187037737986948No Hit
AGACCCGGCGGCGTCCGCCCTCCTCTTGCCGCNNN1006160.13152527647157955No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN1003640.13119586196821192No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGANNN1000240.13075141382874764No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN987560.12909388370862795No Hit
AAGGCGCTTGAGAGAACTCGGGTGAAGGAACNNNN984800.12873309639541577No Hit
ACCTGCATTAAAAATTTCGGTTGGGNNNNNNNNNN975530.12752132161517055No Hit
GCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNNN952730.12454090467993956No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAANNNNNNN948470.12398403730519904No Hit
GAATACAAGCTTGGGCTGCAGGTCGACNNNNNNNN929860.12155133734183725No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACANNNNN923350.12070034987480419No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAANNNNNNN917590.1199474024385353No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCNNN900890.11776437775351963No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN881450.11522318015611213No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN850110.11112641407057973No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN841590.11001267932109868No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN839160.10969502962142273No Hit
GAGAGAGAGACGGGGAGGGCGGCGCCGCCGCNNNN824000.10771331379957616No Hit
ATAAAGTATAGGCGATAGAAATTGAAACNNNNNNN808950.10574597718224167No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGANNNNNNNN800480.10463877843481158No Hit
AGTTCAACTTTAAATTTGCCCACAGAACNNNNNNN792780.10363223411896602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)