FastQCFastQC Report
Sat 16 Sept 2023
SRR15513153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155854461
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN124934498.016099712410542No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN62707844.023487014593698No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN55537783.5634385851810815No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN37448592.402792307626023No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN32826502.106227809545984No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN30096801.9310836409103491No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN29407661.886866748074667No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN26324901.6890693940419195No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN24355571.5627124076993855No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21950461.408394720251222No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGANNN18802391.206406918310795No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN15479480.9932009581682747No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN12737190.8172489846152046No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN9212820.5911168625452434No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN8971670.5756440940115279No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN7758490.4978035245330578No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN7075930.4540088204469168No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN7015640.45014046790742807No Hit
AGCCCGTGGACGGTGTGAGGCCGGTNNNNNNNNNN5623710.3608308651492497No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGANN5214880.3345993413688685No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN4864620.31212581075879503No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNN4514060.28963303142153884No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN4460260.2861810930134364No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN4336140.27821725295370275No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTNNNN4214180.2703920037296847No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN4021180.25800865590879685No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN3811370.24454673774143687No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN3663740.2350744390948168No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNNN3441480.22081369874937365No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNN3409080.21873483621363907No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN3290650.2111360803461378No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN3250000.20852787781287827No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN3085780.19799112455305337No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2928040.18787014380037542No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNNN2642270.16953444791034888No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN2601320.1669069966499066No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN2549230.16356477598674574No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN2533280.1625413853248641No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNNN2432320.15606354700363692No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN2422380.15542577250964926No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN2402420.15414509052775846No Hit
CGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNNN2370840.1521188411796567No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCNNN2221440.14253297504265855No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN2097160.1345588689950941No Hit
AGATCAGACGTGGCGACCCGCTGANNNNNNNNNNN2051930.13165680256017823No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN2043050.13108704023556952No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCNNN1999840.12831458189701736No Hit
ACCGCCTGGGAATACCAGGTGCTGTAGGCTTNNNN1988750.1276030206154959No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTNNNNNNNN1913950.12280367130460257No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN1852400.11885447411094636No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN1779500.11417703340554367No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTNNNNN1753840.11253062560718104No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN1742920.11182997193772978No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN1726000.11074434372462397No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN1713890.10996733677068121No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1695770.10880471364884449No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1679960.10779030572631476No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTNNNNNNNNN1658250.10639733950252472No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGANNNNNN1652980.10605920352834816No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN1650610.10590713858360461No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN1615100.10362873090940913No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNNNN1571990.10086268881325122No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN1562380.10024608791916455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)