FastQCFastQC Report
Sat 16 Sept 2023
SRR15513154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102941473
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN100493489.7621956507267No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN69477116.749185529917567No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN45525924.42250520351501No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN33202963.2254211089441083No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN27799162.700482049639993No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN25111342.439380287476555No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN22941292.228576037570397No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN17338221.6842793768843778No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN15818411.5366411164526468No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN14697871.4277889728661646No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN9909960.9626790555056464No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN9490780.9219588299460217No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN9450420.9180381555255188No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9352140.9084909830268311No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN7832840.7609022653095318No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN7602990.7385740439132826No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN7496620.7282409879641026No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN7334520.7124941761810616No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGANNN5696920.5534134915671938No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN5381520.5227747226814988No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN5209050.5060205423716834No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN5106150.49602457116579246No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN4964700.4822837536043418No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN4198760.4078783679343698No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN4090050.3973179983542688No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN4088180.3971363417346865No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN3577310.34750911326089146No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN3480580.3381125117570447No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN3381720.32850899656351334No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNN3344380.32488169272650685No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN3311370.3216750162492818No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN3230380.31380743891240026No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN3015150.2928994419965217No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGANN2907580.2824498149545616No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN2823090.27424223859707153No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2565310.2492008250163663No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN2544480.24717734513085896No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN2395360.23269144400138903No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN2376150.23082533509113473No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN2336550.22697848902939244No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN2335180.22684540369846853No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN2228330.21646571931217656No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN2130830.2069943180237959No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN2087010.20273753028577707No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN2016610.19589869284267966No Hit
ACTAAAATTGGAACGATACAGAGAANNNNNNNNNN1939490.18840705728001386No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN1929490.1874356315068466No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN1839630.17870639950916575No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN1809930.17582126496285905No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN1745440.1695565401517035No Hit
GTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGNN1734630.1685064288909097No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN1719580.167044433102293No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN1702160.16535220940543566No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNN1682470.16343947205806933No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN1665370.16177833398595334No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN1515530.14722249020081538No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN1436100.13950645528454797No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGN1420830.13802308812892158No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN1417750.13772388899078605No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNNN1408490.13682434872483318No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGNNNNN1322210.12844288715394622No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN1142100.11094653755343097No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN1124770.10926305668853213No Hit
CTAAAATTGGAACGATACAGAGAANNNNNNNNNNN1124170.1092047711421421No Hit
TACTAAAATTGGAACGATACAGAGAANNNNNNNNN1108150.10764854705352818No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNN1042880.10130805103206557No Hit
CGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNNN1037610.10079610964960643No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNN1030940.10014816865890389No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)