FastQCFastQC Report
Sat 16 Sept 2023
SRR15513157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86157567
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN920906910.688636321404015No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN51156855.937592225648619No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN44104135.1190082932587915No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN28518203.310005260478165No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN25931933.0098261711591743No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN22746112.6400594622176365No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN20642432.3958928645234376No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN15244351.7693570664547666No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN15207771.7651113569629933No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN14726741.7092799289469256No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN8556790.9931559464765295No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7948770.9225852443117388No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN7800390.9053633095279954No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN7367330.8550995874802267No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN7328700.850615941835962No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN6573800.7629974045112021No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN6318870.7334085931186984No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN6004390.6969080266623592No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN5820710.6755889473991298No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN5377620.6241610792003911No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGANNN5109870.5930842963566972No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN4465640.5183108292739975No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN4458930.5175320236236476No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN3422440.3972303442598374No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN3357380.3896790632446712No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN3347630.38854741569013895No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN3276960.3803450020820574No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN3196630.37102138689687No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN3043870.353291081211706No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN2932100.34031833791221144No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN2753670.31960860733219176No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN2745880.3187044499527244No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNN2706850.3141743777421198No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN2605930.30246095505459203No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN2567070.2979506141346819No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN2485540.2884877192504751No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGANN2440470.2832566058881398No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN2353760.27319248696983284No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN2230760.25891631782034885No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN2212130.25675400049307334No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2159980.25070113690652385No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN2104950.2443140020423279No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN2069540.24020409025709835No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN2022830.23478262797276991No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN1836510.21315713337169795No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN1823270.21162041402585102No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN1785650.20725399546159423No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN1716850.19926862605115112No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNN1641900.19056944818323387No Hit
ACTAAAATTGGAACGATACAGAGAANNNNNNNNNN1620140.18804384297434953No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN1517730.17615748132720602No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN1496890.1737386572209032No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN1430860.16607479178236312No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN1423750.16524955956567344No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGNNNNN1335740.15503455430676216No Hit
GTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGNN1290930.1498336182125477No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN1288060.14950050759905975No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN1256770.14586878944713003No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN1239930.14391423100422507No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN1226330.14233572774867237No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN1224630.1421384148417283No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNNN1143920.1327706944185181No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGN1096950.12731905486606881No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN1059290.12294799364517801No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNNN1050600.12193937649144619No Hit
CGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNNN963050.11177776178382569No Hit
CTAAAATTGGAACGATACAGAGAANNNNNNNNNNN906660.10523277659407444No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNN878540.10196898898038752No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNN867890.10073288165159075No Hit
TACTAAAATTGGAACGATACAGAGAANNNNNNNNN863460.10021870742937763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)