FastQCFastQC Report
Sat 16 Sept 2023
SRR15513158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68655478
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN783511211.412216808103791No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN37768145.501110923734301No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN35269165.137122488608994No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN22342873.2543462882888967No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN20657143.0088116202468216No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN17936232.6124980150891965No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN16363582.383433991967837No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN13119001.9108453370610863No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN12108401.7636465949592544No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN11876031.7298007888023152No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN6954371.0129373798839474No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6320240.9205733007932738No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN6061150.8828355983480298No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN5376720.7831450827565428No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN5274810.7683014019653319No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN5052350.735899034888374No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN4998760.7280933940915829No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN4809510.7005282229627765No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN4732950.6893768913822143No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN4260390.6205462585228815No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGANNN4003990.5832003674928896No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN3534620.5148343734494136No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN3465860.5048191493182816No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN2767950.40316520700649694No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN2669330.3888007305112638No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN2664590.3881103267535331No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN2622040.3819127149620894No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN2592770.3776493989307015No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN2552410.37177077115390555No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN2326260.3388309378604865No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN2192930.3194107832152884No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN2174340.31670306046081276No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNN2162070.3149158760499781No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN2083210.30342953842663506No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN1993990.2904342170627666No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN1901290.2769320169906908No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGANN1899660.2766945996647201No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN1887070.2748608057174986No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN1845400.2687913701511189No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN1766530.2573035759797638No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN1712110.2493770416979691No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN1662870.24220499928643713No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1627370.23703425384351703No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN1622910.23638463343012484No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN1475630.2149325943080609No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN1445360.21052362347546397No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN1411680.2056179697707443No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN1375580.2003598314470988No Hit
ACTAAAATTGGAACGATACAGAGAANNNNNNNNNN1296610.18885747179562276No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNN1277230.18603468174819204No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN1214120.176842407243891No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN1175290.1711866313129449No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN1121290.163321272047658No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN1117780.16281002369541436No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN1043630.1520097201857658No Hit
GTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGNN1025310.14934132422761662No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN1022430.14892183840013465No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGNNNNN1014990.14783816667913957No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN963870.14039229324133465No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNNN908070.13226475533387153No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN883510.1286874734161781No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGN860650.12535780466053997No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNNN851050.12395951856893342No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN840510.12242431696419039No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN839760.12231507586328363No Hit
CGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNNN768570.11194591056521373No Hit
CTAAAATTGGAACGATACAGAGAANNNNNNNNNNN731070.10648385551987563No Hit
TACTAAAATTGGAACGATACAGAGAANNNNNNNNN705490.10275800570494899No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNN688530.10028770027644407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)