FastQCFastQC Report
Sat 16 Sept 2023
SRR15513159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62564469
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN41488596.63133415229657No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN20210153.2302919409417505No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN19917003.183436272750912No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16354582.6140364109859227No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN13876422.217939386650912No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN10597331.6938256121058104No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN9788871.5646053033711513No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTNNNN9232601.475693815926097No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN8770581.4018467894293165No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN7979761.2754459723777085No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN7948721.2704846899603672No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN7666701.2254079867600252No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN5672910.9067303040644363No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN4055070.6481426382760477No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN3555510.5682954010206656No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN3418790.5464427421257263No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN3393930.5424692408082293No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN3194430.5105821324880101No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN3155040.5042862267399728No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAANNNNNNN2842200.45428340485076285No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN2828310.45206329490305436No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACNN2481410.39661648850564046No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN2332440.3728058492752492No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN2020960.3230204031620567No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATNNNNN1958210.3129907487906594No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN1928280.30820688336697943No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN1904030.3043308814784315No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNNN1713070.2738087651634988No Hit
GAATACAAGCTTGGGCTGCAGGTCGACNNNNNNNN1701160.27190512877205114No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN1678960.2683567888988237No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN1478680.23634500917765322No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN1375610.21987080238785373No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN1318120.21068188079723013No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN1317300.21055081599110193No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN1274260.20367151202066464No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN1263600.20196766954099776No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN1247850.19945026625255943No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN1226850.1960937285346416No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN1221520.1952418072948082No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1178810.18841524891708103No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN1120760.17913681965397965No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN1102870.1762773691885725No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN1078820.1724333343259095No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNN1072190.171373627417824No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN1030910.16477563327517414No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATNNNNNN1030030.16463497836128044No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN1015830.1623653195234503No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN1011370.16165245484621632No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN988860.15805456608286728No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN961920.15374860769616697No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN958510.1532035699048289No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN946590.15129833516208696No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN941390.15046719248907875No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNN936400.1496696151932497No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN927210.1482007303538371No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN902660.14427677792646174No Hit
AATCATGGCCTCAGTTCCGAAAACCAACNNNNNNN889610.14219092948746995No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCNNNNN886690.14172421090954995No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN885670.14156117907753682No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN866490.13849554129517186No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN857780.13710337731788308No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN844630.13500154536594886No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN841430.13449007295178994No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN835050.1334703248260606No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN799180.12773703873359812No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN797290.12743495033898553No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGANNNNNN793920.12689630595282445No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNNN791840.12656384888362116No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN790660.12637524343090006No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN767420.12266067502307101No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN755300.12072347325444416No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN748210.11959024218682332No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN743860.11889495937382605No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGNNNNN730790.11680591423224577No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCNNNN723980.11571743700086387No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNN720940.1152315382074129No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCNNN719310.11497100694645071No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN717690.11471207403678275No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGNNNNNNN685130.10950784222271591No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGNN682130.10902833683444192No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN676690.10815883373037179No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN670670.10719662625123534No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN668070.10678105491473125No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN666030.10645499125070493No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN658110.10518909702566163No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTNNNNN657430.10508040913765287No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTNNNN642980.10277079151746657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)