FastQCFastQC Report
Sat 16 Sept 2023
SRR15513160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50307263
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN14716072.925237654054048No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN10808012.1483995263268447No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN8279371.6457603746003833No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN7580111.5067625523574995No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN7534241.4976445846397966No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTNNNN6867371.3650851965450794No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN6631191.3181377011108715No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN5596541.1124715729416645No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN4917460.977485099914897No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4765700.9473184816275931No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN3987230.792575417986862No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN3878970.7710556624795907No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTNNNNN2591110.5150568417924068No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACNN2536690.5042393182869042No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN2330780.4633088466768705No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN2235030.44427580963806357No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN2232160.4437053154730362No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN2010420.39962818092488955No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATNNNNN1935540.38474365023595103No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN1893580.3764029062761773No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN1652130.3284078483856297No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN1648120.327610746782229No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN1573400.3127580206460447No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN1421270.282517854330497No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN1387060.27571764339475197No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN1292260.25687344588792277No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN1291800.25678200779875465No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN1196420.2378225187881917No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN1188540.23625614456505017No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN1168870.23234617236083782No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN1160970.23077582256860207No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN1137310.22607272433008332No Hit
AGCTGCGAGAATTAATGTGAATTGCAGNNNNNNNN1135330.2256791429897508No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN1118580.22234960387330158No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1094860.21763457892750002No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN1055390.20978879331996259No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN1026920.2041295707142724No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGANNNNNN1014760.2017124247049576No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN990860.19696161963730763No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN966540.19212732761867804No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTNNNNN932130.18528736099199036No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN909480.18078502899273213No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATNNNNNN859320.17081430170430859No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN840010.16697588974379304No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN831480.16528030952508785No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN824790.1639504816630553No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN815360.16207600083510804No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN801870.15939447948102445No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGNNNNNNN759960.15106367444398636No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN757560.15058660615267422No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCNNN750090.1491017310959652No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN743580.14780768335578104No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCNNNNN741090.1473127250035447No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGNNNNNN735730.14624727248628094No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN731980.14550185328110576No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTNNNN730670.14524145350543122No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNNN708910.14091603433086788No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN695220.1381947572858416No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN693930.13793833307926134No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCNNNNNNN693030.1377594324700193No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN684860.1361354124950109No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGNNNNNNN603430.11994888292769972No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAANNNNNNN581050.11550022111121411No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNN580760.11544257535934722No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTNNNNN550800.10948717285613412No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN521460.10365501299484331No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNN518970.10316005464260697No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNN517920.10295133726515793No Hit
AACCTATTCTCAAACTTTAAATGGGTNNNNNNNNN514180.10220790584452985No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNNN509930.10136309741199795No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATN505320.10044672873576924No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)