FastQCFastQC Report
Sat 16 Sept 2023
SRR15513162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95283260
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN31216713.276200877257978No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN19946972.0934390783858574No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN17583721.8454154486317955No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN14015591.4709393864147804No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN13984751.4677027213384597No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTNNNN12561411.3183228617492726No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN11117231.1667558393782915No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN9965671.0458993531497558No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8872200.9311394257501265No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN8661870.909065243989343No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN8464730.8883753557550402No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN6834830.7173169767701063No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTNNNNN4694580.4926972481839937No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACNN4539710.4764436061486561No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN4390860.46082176449462375No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN4335130.4549728882072255No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN4154600.43602622328413193No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN4136030.43407729752319557No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATNNNNN3527180.3701783503209273No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN3517610.36917397662506507No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN3138520.3293883941418461No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN3109690.32636267902672517No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN3003120.3151781330739523No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN2826340.29662503151130637No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN2640190.2770885462986888No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN2632080.2762373999378275No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN2300240.24141071579624795No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN2191640.23001312087768616No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN2191470.22999527933867922No Hit
AGCTGCGAGAATTAATGTGAATTGCAGNNNNNNNN2140430.2246386196274141No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN2125970.22312103930952823No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN2122520.22275896101791648No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN2082060.21851267473426078No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN2029410.21298704515357683No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN1971680.2069282684072732No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN1963990.20612120114278207No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN1924870.20201554816659295No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGANNNNNN1897120.1991031792992809No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN1865090.19574162344991133No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN1819470.1909537939822798No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTNNNNN1812960.19027056798854278No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1718030.18030764270659924No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN1596690.16757298186481026No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN1590900.1669653200362792No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATNNNNNN1590770.16695167650645035No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN1586150.16646680644637893No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNNN1572360.16501954278222639No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN1560770.16380316962286973No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN1535710.1611731168727854No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN1442220.15136131992125373No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN1417050.14871972264592961No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGNNNNNNN1413390.14833560480613278No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCNNN1365600.1433200333405889No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCNNNNN1362760.14302197468894326No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN1334900.1400980612963914No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTNNNN1333860.13998891305776062No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGNNNNNN1327170.13928679602272215No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN1291030.13549389473030204No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN1289070.1352881922805748No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCNNNNNNN1275100.13382203757512076No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN1232980.12940153391057357No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGNNNNNNN1136130.1192371041880809No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNN1065640.111839162513961No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAANNNNNNN1048470.11003716707425838No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN988990.10379472742641258No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTNNNNN971960.10200742501883331No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNN969210.10171881188783843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)