FastQCFastQC Report
Sat 16 Sept 2023
SRR15513163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119191270
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN81171606.810196753503843No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN43118533.6175912883552632No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN42535163.5686472675389735No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30483832.557555599499863No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN27762242.3292175676960234No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN19878241.6677597277048897No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN18430721.5463145916643055No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN18293851.5348313681027141No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTNNNN16909271.4186668201454689No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN15882011.3324809778434277No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN15629101.3112621419337172No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN14437371.2112774702375433No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN10874280.9123386301698103No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN7132120.5983760387820349No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN6874810.5767880483193106No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN6303990.5288969569667309No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN6245410.5239821674859241No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN6030490.5059506455464398No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN5894640.494552998722138No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAANNNNNNN5225740.43843311678783187No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN4991830.4188083573570447No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACNN4606400.3864712575006542No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN4370560.36668457345911326No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN3788040.3178118665905649No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN3662900.30731277550780356No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATNNNNN3623000.3039652149020646No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN3447550.2892451771006383No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNNN3384510.2839561991410948No Hit
GAATACAAGCTTGGGCTGCAGGTCGACNNNNNNNN3165390.2655723024010064No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN3088180.2590944789832343No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN2705730.2270073974377486No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN2595090.2177248384046919No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN2521300.21153394875312598No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN2408660.20208359219597208No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2402350.20155419100744543No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN2395330.20096522169786427No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN2378290.1995355867925562No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN2348120.19700436114155007No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN2330460.19552270900377186No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN2241880.18809095666150719No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN2236500.18763958132168573No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN2067300.1734439107830632No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN2063830.1731527820787546No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNN1987040.16671019614104288No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATNNNNNN1938320.16262264845403526No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN1922680.1613104718155952No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN1898720.15930025747691084No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN1871390.15700730431012272No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN1868240.15674302320966962No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN1842060.1545465536192374No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1821850.15285095963823525No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN1791380.15029456435861452No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNNN1789310.15012089392117392No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNN1779380.14928777921403139No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN1731630.14528161332621087No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN1717110.14406340330126527No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN1698950.14253980178246276No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN1681730.14109506509998593No Hit
AATCATGGCCTCAGTTCCGAAAACCAACNNNNNNN1679700.14092475061302728No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN1658710.13916371559762725No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN1658650.13915868167190432No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCNNNNN1640650.1376485039550296No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN1588510.13327402250181578No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN1584370.13292668162693458No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN1577630.13236120397072704No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN1566910.13146180924156609No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGANNNNNN1521930.12768804292462024No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN1511990.1268540892298572No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN1493410.12529525023099428No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN1474340.12369530083872753No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN1440000.12081421734997874No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGNN1417700.11894327495629503No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN1401600.11759250488731264No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN1399240.11739450380887795No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCNNNN1353400.11354858455657028No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNN1350080.11327004066656896No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN1349960.11325997281512311No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGNNNNN1338200.11227332337343163No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCNNN1332600.1118034903059595No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGNNNNNNN1305530.10953235081730399No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN1289510.10818829264928548No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN1256890.10545151503126027No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN1246000.10453785751255104No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN1239230.10396986289348203No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTNNNN1238980.1039488882029699No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)