FastQCFastQC Report
Sat 16 Sept 2023
SRR15513164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124802981
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN38069723.0503854711611416No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN26363062.1123742228561033No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN21317911.7081250647370354No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN18540061.4855462466878095No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN18319021.467835131277834No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTNNNN16342761.3094847470029582No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN14967831.1993167054238874No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN14210861.1386635067635125No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11722140.9392516032930336No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN11310880.9062988647683022No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN10241320.8205989887372962No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN8896060.7128082942185492No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTNNNNN6404400.5131608194519008No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACNN6104780.4891533800783172No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN5691270.4560203573983541No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN5601520.4488290227618842No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN5514220.4418339975388889No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN5447630.43649838780693867No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATNNNNN4718400.37806789246484424No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN4693300.3760567225553691No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN4081780.3270578929520922No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN4073440.32638963968336626No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN3847160.3082586625074284No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN3805490.3049197999525348No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN3629450.29081436764719587No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN3454020.2767578123794976No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN3149090.2523249023995669No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN3013110.24142932932026678No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN2862090.22932865682110587No Hit
AGCTGCGAGAATTAATGTGAATTGCAGNNNNNNNN2819800.22594011596565952No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN2772390.22214132849919668No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN2767910.22178236271455729No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN2749840.22033448063231761No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN2680960.21481538169348693No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN2650370.21236431844524611No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN2569920.20591815831706778No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN2557270.20490456073320876No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGANNNNNN2504710.20069312286699306No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN2448880.19621967202850704No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN2347580.18810287872851372No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTNNNNN2334940.18709008240756686No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN2235130.17909267728148257No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATNNNNNN2131500.17078918972296023No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2090860.16753285724801717No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN2051260.16435985611593684No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN2049340.16420601363680568No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNNN2023760.16215638310754774No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN1997800.1600763045876284No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN1958800.15695137923027655No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGNNNNNNN1862400.14922720475723253No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN1854510.14859500831955288No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN1835030.14703414816670124No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCNNNNN1807960.14486512946353422No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGNNNNNN1805000.14462795564154032No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCNNN1802520.14442924243932925No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN1760870.1410919824102599No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTNNNN1746070.13990611330029049No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN1727690.1384333920677744No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN1715760.1374774854135896No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCNNNNNNN1703550.1364991433978648No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN1676200.13430768933315784No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGNNNNNNN1498140.12004040191956633No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNN1420830.11384583834580041No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAANNNNNNN1406030.11265996923583098No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN1298570.10404959798195847No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTNNNNN1284240.10290138822885969No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNN1274980.10215941877221665No Hit
AACCTATTCTCAAACTTTAAATGGGTNNNNNNNNN1266510.10148074908563282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)