FastQCFastQC Report
Sat 16 Sept 2023
SRR15513170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29820720
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length35-50
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN15228475.1066741513954055No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN14827674.972270957911143No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN13621824.567904463741989No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN9825723.2949305046960635No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN9580883.2128265179378634No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN9541083.199480093035983No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7635052.5603171217864626No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN6609732.216489072027771No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN6242132.0932190772053794No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN5155701.7288985644880472No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN4009481.3445282340600764No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN3100691.0397770409299305No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN3069751.0294017045866097No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN2882760.9666969811594086No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN2410500.8083305835673987No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN2272520.7620607416588198No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN2169520.7275209988222954No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNN1763360.5913203973612978No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN1703880.5713745342164777No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN1647430.5524447431182077No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1575440.5283038102366409No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN1532550.5139211930496648No Hit
TCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNN1425110.47789255256076985No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN1386120.4648177508792544No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN1334200.4474070377911734No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN1321850.4432656220238813No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGNNNNNNN1251480.4196679355830442No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN1248790.4187658782215855No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN1215570.40762597281353363No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN1193560.4002451986404084No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN1189630.398927323015675No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN1165190.39073167918145507No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN1160310.3890952331130838No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN1124040.3769325489122999No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN1100810.36914266322208183No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN1066530.35764730026639197No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN947420.3177052733803879No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN913610.30636751895997144No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN892090.2991510600683015No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN877600.2942920224595516No Hit
AGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNN873460.2929037259999088No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN851780.28563361313878405No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN839300.28144860352131No Hit
ACGATGCCGACCGGCGATGCGGCGGCGNNNNNNNN824840.2765996260318329No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN810090.271653400722719No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN773810.25948736314884413No Hit
CGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNNNN760130.2548999487604592No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN729480.24462186023677499No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN706510.23691916224692094No Hit
CGGCCGAGGTGGGATCCCGAGGCNNNNNNNNNNNN700130.23477971021491098No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN699910.23470593600691064No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN619830.20785212429478567No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNNN610340.2046697732314981No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN596310.19996499078493074No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGANN583350.19561901925909234No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN552430.18525038966195315No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNN528630.17726936170555238No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCNNNNNNNNN502460.16849358432660244No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN499070.16735679084877897No Hit
ATTGATCATCGACACTTCGAACGCANNNNNNNNNN478140.16033818096947358No Hit
CGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNNN428960.1438462921083059No Hit
CCGGCCGAGGTGGGATCCCGAGGCCNNNNNNNNNN428810.14379599151194203No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN422980.14184097499993295No Hit
ATTGATCATCGACACTTCGAACGNNNNNNNNNNNN414750.1390811489461019No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN407400.13661641972427227No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN394590.13232074879479772No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNNN378810.12702912605731853No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN375930.12606335460713222No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN343530.11519842579253621No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN327280.10974919451978354No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN320140.10735488613286333No Hit
CCGGCCGAGGTGGGATCCCGAGGCNNNNNNNNNNN313960.10528250156267185No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN312470.10478284897212407No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN311580.10448439876703179No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGANN302070.10129534095756239No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNN301070.10096000364846992No Hit

[OK]Adapter Content

Adapter graph