FastQCFastQC Report
Sat 16 Sept 2023
SRR15513171_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513171_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33002482
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length35-50
%GC70

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23864287.231056136929339No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN18381005.569581099991207No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN15359234.653962086851529No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN14497464.3928393022076335No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN9696252.9380365997927065No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN9184492.7829694748413165No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN8207842.4870371870818686No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN6487641.9658036628881426No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN3881381.176087301555077No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN3805871.1532072042339119No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN3068830.9298785467105171No Hit
CCGGCCGAGGTGGGATCCCGAGGCCNNNNNNNNNN3064120.9284513813233805No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN2928050.887221149003278No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN2808890.8511147737312605No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN2459770.7453287907254975No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN2334110.7072528666177289No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN2260160.684845461017144No Hit
CGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNNNN2200290.6667044012023096No Hit
CGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNNN2195450.6652378448384579No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN1954740.592300906337893No Hit
TCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNN1847670.5598578919003728No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN1804050.5466407041749163No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1776350.5382473960594842No Hit
CGGCCGAGGTGGGATCCCGAGGCNNNNNNNNNNNN1684350.5103707048457748No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN1638300.4964172088632607No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN1612760.4886783969763244No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN1536020.4654256004139325No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN1404860.42568313498360516No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCNNNNNNNNN1355950.41086303751336034No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGNNNNNNN1290360.3909887747230648No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNN1260620.38197733128072003No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN1232270.37338706828171286No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN1200050.3636241662066508No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN1185260.3591426850865338No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN1181570.35802458736285353No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN1071210.32458467820693No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN1043520.3161944001666299No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN1024460.310419076965181No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN1018750.3086889040648519No Hit
AGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNN1011080.30636483643866547No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN952700.2886752578184877No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN923670.27987894971050964No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN911200.27610044602099926No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN852300.2582533034939615No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN799610.24228783762384903No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN777340.23553986030505222No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNNN743350.22524063493163937No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCNNNNNNNN732530.22196209363889663No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN715360.21675945463738153No Hit
ACGATGCCGACCGGCGATGCGGCGGCGNNNNNNNN701130.21244765772465232No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN689840.20902670290070913No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN680730.20626630445552552No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN658530.19953953766265217No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNN643080.19485807158382815No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN638390.19343696634695537No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN634110.1921400941904915No Hit
CGGCCGAGGTGGGATCCCGAGGCCNNNNNNNNNNN607410.18404979358825194No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN604670.18321955300210452No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN594070.1800076733622641No Hit
CCGGCCGAGGTGGGATCCCGAGGCNNNNNNNNNNN562260.1703690043676109No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN535840.16236354586906523No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN523690.15868200458377646No Hit
CGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNN506010.15332483175053319No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN495490.15013719271174816No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN446010.13514438095898362No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN442100.133959621582401No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTNNNNNNN420060.12728133599163846No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN391570.11864865194078433No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNNN375430.1137581106778575No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNN374050.11333996030965186No Hit
CGATGCCGACCGGCGATGCGGCGGCGNNNNNNNNN369260.11188855432145983No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN364400.11041593780734432No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN354320.10736162207436398No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN348500.10559811834758369No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)