FastQCFastQC Report
Sat 16 Sept 2023
SRR15513172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30492941
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length35-50
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27942279.163520829296196No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN15956865.2329685090067235No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN13175444.32081641452689No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN12457684.085430788719265No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN9138652.9969723156582373No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN7097822.327692825693658No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN6411322.102558752860211No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN4475531.4677265797352903No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN3960141.2987071335624858No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN3122461.0239943729927525No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN2776050.9103910311570143No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN2369930.7772061081284354No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN2242550.7354325055100457No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN2049660.6721752421322692No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN1981020.6496651142964531No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN1859630.6098559007476517No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN1537130.5040937179526238No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN1521020.4988105279841653No Hit
ATTGATCATCGACACTTCGAACGCANNNNNNNNNN1452180.4762348111977785No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNN1410610.4626021478216877No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN1404390.4605623314589432No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN1322790.43380203962615477No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN1242680.407530385475117No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG1228810.4029817917530487No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN1212200.3975346294081637No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNN1158720.37999614402559595No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN1121050.367642465185631No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1114530.36550426539703074No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN1008310.33066997374900636No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN987410.3238159284143829No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNN973650.3193034086151283No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN973630.3192968497200713No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN946250.31031772238696165No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN859720.2819406629226089No Hit
ATTGATCATCGACACTTCGAACGCACNNNNNNNNN850390.27888093837849226No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN812980.26661252517426903No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNN812710.2665239800909988No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC801030.26269358537767806No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATN800170.26241155289022466No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNN792780.2599880411666425No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN792500.25989621663584367No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN790010.2590796342012402No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGANN741580.24319726982057915No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN714070.23417550966959863No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN692490.2270984619030352No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNN688970.2259440963729933No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN647300.21227863852161716No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN645740.2117670447071668No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN640380.21000926083187582No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN628920.20625101396418272No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN607260.1991477306173911No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN599280.19653073148962574No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN593030.19448107678429577No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN588910.19312994440254222No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN587520.1926741011960768No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN552530.18119931429375738No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNNN533480.17495196675191152No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN494260.16208997354502472No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN487720.15994521486136742No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN478810.157023227113449No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN477880.15671823849329589No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG457670.1500904750381408No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNNN454450.1490344929339548No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN451730.14814248320619516No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN451720.14813920375866665No Hit
TTGATCATCGACACTTCGAACGCACNNNNNNNNNN414790.13602820403581273No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN407700.1337030757380864No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN407520.13364404568257288No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN402010.13183707009435397No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN377250.12371715801371866No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN374070.12267429369964675No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN371590.12186099071257181No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNN370450.12148713369431961No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAN365350.11981461545477035No Hit
ATTGATCATCGACACTTCGAACGCNNNNNNNNNNN362670.11893572351712484No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNN360360.11817817113803487No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN353500.11592847013346466No Hit
TTTTCACTGACCCGGTGAGGCGGGGNNNNNNNNNN351290.11520371222965997No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNNN350480.1149380769798492No Hit
GCGCACCACCGGCCCGTCTCGCNNNNNNNNNNNNN340500.11166518834637826No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGANN329830.10816601783343888No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGN326090.10693950445776942No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNNN309000.10133492863151508No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN305150.10007234133303179No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)