FastQCFastQC Report
Sat 16 Sept 2023
SRR15513173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30440947
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length35-50
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN14502684.764201323960125No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN12904654.239240651744507No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN12823254.21250035355339No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN10604353.483580849176604No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN10464233.4375507437400024No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN8218712.6998864391439596No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7667752.5188933839673253No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN5531771.817213505217167No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN5278311.7339506553459065No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN5085531.6706214823080239No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN4677621.536621051900915No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN3819421.2546981537729427No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN3776551.2406151490622155No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN3628621.1920194204207903No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN3350611.1006917754562628No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN3242311.065114695676189No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN2434020.7995874766970948No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNN2307360.7579790471038894No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN2182120.7168370944570154No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1851820.6083319287011669No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG1847960.6070638998188854No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN1682510.5527127654734263No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN1665320.5470657663836805No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN1654980.5436690258026466No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN1634240.5368558343470721No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN1590900.5226184323372068No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN1485720.48806628781949524No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN1485380.4879545961562891No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN1360820.44703602683582744No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN1258210.41332813988999756No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN1195330.39267175229469703No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN1142360.37527084817696377No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC1126730.3701363167183991No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN1082450.35559012011025803No Hit
ATTGATCATCGACACTTCGAACGCANNNNNNNNNN1017070.3341124702855006No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN871340.28623945240599774No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN763070.2506722277726774No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNNN757110.24871433861765208No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN755350.24813617000811442No Hit
TCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNN702980.2309323688254508No Hit
ATTGATCATCGACACTTCGAACGNNNNNNNNNNNN699120.22966433994316932No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNNN688660.22622817877512155No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN614290.20179726997323705No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN593990.19512862067004683No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNN565510.18577280135207355No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN561550.1844719219806138No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN535900.17604577150638578No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN532670.17498470070592745No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN521180.17121017949934342No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG520650.17103607190669856No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN520360.17094080548808155No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN499040.16393708119527292No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN466330.15319168618505857No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN464270.15251496610798607No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN452980.1488061458797586No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG444250.14593829817449502No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN434920.1428733475341618No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN428180.14065922456354593No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN425570.13980182679599293No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN395020.12976600235202934No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGNNNNNNN393950.12941450211782177No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGANN356000.11694774147466569No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN350580.11516724496120308No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN348880.11460878664517238No Hit
ACGATGCCGACCGGCGATGCGGCGGCGNNNNNNNN343940.11298597247976551No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN337370.11082769534075271No Hit
TCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNNN331910.10903405863161879No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNN325510.10693162732420906No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN325150.10681336556316726No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN321070.10547306560469358No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNN319670.10501315875619768No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN317790.10439556955964609No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTNN309030.10151786670762902No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG306980.10084443167947436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)