FastQCFastQC Report
Sat 16 Sept 2023
SRR15513180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31225498
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length35-50
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN16018135.129823710097433No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15249164.883560223763285No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN10573073.3860372699260073No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN10535273.3739317784459355No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN6470572.072207143021386No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN5538991.773867625746113No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN5462001.749211493760644No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN4645241.4876432074838326No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN3860631.2363709939870295No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN3183801.0196154437632987No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN2517040.8060848220899471No Hit
TCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNN2217550.7101728209426796No Hit
ACGATGCCGACCGGCGATGCGGCGGCGNNNNNNNN2022670.6477622870898648No Hit
AGGGAGGACAGAAACCTCCCGTGGAGCNNNNNNNN1893130.6062769599383171No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN1822680.5837152701295588No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNNN1794810.574789872046236No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNNN1775500.568605823356284No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN1599660.512292870397135No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN1451940.464985378295648No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCNNNNNNNN1181410.37834784892782175No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN1180350.378008382764624No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN1157720.37076110043144866No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNN1148510.3678115878247963No Hit
CGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNNNN1135590.3636739436469516No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGNNNNNNN1108160.35488945604646566No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN1087330.34821862568853185No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN1055640.3380698684133076No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN1051980.3368977493969832No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN1020910.3269475478021199No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN1018980.32632946318422207No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN948690.3038190135510409No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN895820.28688733803380817No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC862500.276216571469893No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN862220.27612690116263316No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN840820.26927352767920626No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN835360.2675249566876403No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNN829050.2655041722633215No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN789920.25297274682376564No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN765610.24518744264703157No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNNN758710.24297771007527244No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN739110.2367007885670871No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN713600.22853118307352535No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNN712530.228188514399354No Hit
ATTGATCATCGACACTTCGAACGCANNNNNNNNNN653600.2093161172321415No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN642340.20571008987590847No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN633750.202959132949617No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCNNNNN626780.20072698280104292No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN604810.19369106619212287No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNN594610.19042450499908758No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCNNNNNNNN588940.18860868127707683No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCNNNN585070.18736930953030756No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN568710.1821300015775569No Hit
ATTGATCATCGACACTTCGAACGCNNNNNNNNNNN555920.17803399004236858No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC551650.17666651785665674No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCNNNNNNNNN540990.17325264115883757No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN490630.15712479589596937No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN490630.15712479589596937No Hit
CGATGCCGACCGGCGATGCGGCGGCGNNNNNNNNN480860.15399594267479738No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN476670.15265409057687407No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN468280.1499671838700539No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN464450.1487406221671789No Hit
ATTGATCATCGACACTTCGAACGNNNNNNNNNNNN443610.14206658929827157No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNNNN440160.140961723012392No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN419810.1344446131811893No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNN416010.13322765901123498No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNN403220.12913164747604666No Hit
CGGCCGGGCGCGACCCGCTCCGGGGACNNNNNNNN401980.12873453611532473No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG396600.12701158521154732No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN364790.1168243978046403No Hit
ATGGCCTCAGTTCCGAAAACCAACNNNNNNNNNNN357760.11457303259022483No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN348000.11144738188002638No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN345150.11053466625256066No Hit
ACCCCTCCCACGGCGCGACCGCNNNNNNNNNNNNN345110.11052185620866639No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN343080.1098717464810329No Hit
AACGATGCCGACCGGCGATGCGGCGGCNNNNNNNN339990.10888217059020164No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNNN338140.10828970606009229No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCNNNN335130.10732575025704955No Hit
GGGAGAGCGACGCTCAGACAGGNNNNNNNNNNNNN316700.10142352253277113No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGC314450.10070295756371925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)