FastQCFastQC Report
Sat 16 Sept 2023
SRR15513182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30286688
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length35-50
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN22638507.474736095277239No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN13427674.433522080724046No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10479293.460031681245569No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN9762943.223508625307594No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN7158052.3634310889325367No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN5374461.7745287962817196No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN4996461.6497214881997002No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN4179251.3798966727560307No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN4018941.3269658273628335No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN3156411.04217734207187No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN2248490.7424020744691529No Hit
TCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNN2020820.6672304347045144No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNNN1769340.5841972552429635No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN1766650.5833090762515862No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNNN1601970.5289353527199805No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN1572990.5193667924336923No Hit
ATTGATCATCGACACTTCGAACGCANNNNNNNNNN1448490.47825962350191614No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN1441120.4758262111723804No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN1439710.4753606601025507No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN1415520.4673736527414289No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNN1307250.4316252737836504No Hit
ACGATGCCGACCGGCGATGCGGCGGCGNNNNNNNN1225080.40449454228867815No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN1224860.4044219031146622No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNN1216160.4015493539603935No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN1205690.39809238963335974No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN1186680.39181570464225074No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNN1062710.35088353008424034No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGNNNNNNN1051730.347258174944715No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN1040190.3434479200895126No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN1029340.33986548809826944No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN995070.32855028585496043No Hit
CGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNNNN982450.32438343869095226No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNN978640.3231254602682208No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN971990.32092977614455565No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN954390.31511864222327646No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN950590.31386396558118207No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN911390.3009209854837875No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNNN902660.29803853098760746No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN840820.27762031952784005No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC835250.2757812277129807No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCNNNNN834620.27557321553284403No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN804940.2657735306019595No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN802960.2651197780358156No Hit
ATTGATCATCGACACTTCGAACGCACNNNNNNNNN790780.26109820921983945No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN761790.25152634715291416No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN750010.24763684956242163No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN740510.24450015795718566No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN683300.22561067093239115No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCNNNNNNNNN650480.21477422688146028No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN645570.21315305258864883No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNNNN640550.2114955587088294No Hit
ATTGATCATCGACACTTCGAACGNNNNNNNNNNNN627810.20728909017717617No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNNN567890.18750482059973014No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN541580.17881783574354515No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN528370.17445618352194867No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN494610.16330937209113125No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC477790.1577557770595451No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN459940.15186209862233865No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN459430.15169370780984703No Hit
AGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNN455270.1503201670648174No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN447170.1476457247487741No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN441160.14566135458588275No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN424180.14005493106410313No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN409000.13504282805699983No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN394170.13014628737219466No Hit
CGATGCCGACCGGCGATGCGGCGGCGNNNNNNNNN393010.1297632808182922No Hit
CTCCATCTAAGGCTAAATACCGNNNNNNNNNNNNN389620.12864397718231851No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNN388190.1281718225512146No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN373240.12323566049876435No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN340820.11253128767331708No Hit
ATTGATCATCGACACTTCGAACGCNNNNNNNNNNN321480.10614564392118413No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN314930.10398297760388986No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNNN312070.103038668341682No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN309790.10228586235642538No Hit
TTGATCATCGACACTTCGAACGCACNNNNNNNNNN309620.10222973208559483No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN306920.1013382513135804No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN306780.10129202638466114No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCNNNNNNNN306090.10106420352070189No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNN304000.10037413136755No Hit

[OK]Adapter Content

Adapter graph