FastQCFastQC Report
Sat 16 Sept 2023
SRR15513191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30693906
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length35-50
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN20311756.6175187999859No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN19336716.299853136971229No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17936335.843612735374898No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN15529625.059512464786984No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN11408093.7167280045752404No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN10554983.4387868393159216No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN6286982.048282808971918No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN4164381.356744886102147No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN3654621.190666316629757No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN3396721.1066431232310414No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN3339241.0879162788861085No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN3069561.000055190108421No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN2877570.9375053145728667No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN2755280.8976635296921807No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN2747430.8951060187647673No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN2631330.8572809208446784No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG2563120.8350582685696634No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN2032000.6620206629941461No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN1867840.6085377338420206No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN1821510.5934435324067259No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN1527180.49755153351938985No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN1451940.47303852432466564No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN1448220.47182655736288504No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN1412730.46026400159041336No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1324610.431554719689309No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN1322960.43101715369819665No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNN997260.324904885028318No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC885730.2885686820048253No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN867150.2825153631473296No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN865960.28212766403858797No Hit
ATTGATCATCGACACTTCGAACGCANNNNNNNNNN841920.2742954904468659No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN827590.26962681126344756No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN723830.23582205536173856No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN695610.22662804792586516No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN636340.20731802592996798No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN617760.20126470707247232No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN599340.195263515826236No Hit
ATTGATCATCGACACTTCGAACGCACNNNNNNNNN572100.1863887900093263No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN553420.1803028913947935No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN525460.17119359132721654No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN519060.1691084868768413No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN518150.16881201108780355No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGANN493510.16078435895385879No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN465050.1515121601010963No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN456690.1487884924127936No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN455790.14849527459945958No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN453370.14770684447916144No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN448900.14625052933960245No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN445530.14515259152745175No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN439490.14318477420241008No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN427240.13919375396536368No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN407960.13291237680860818No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN396340.1291266090408956No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN394170.12841962831319026No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN384200.12517142653659002No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN374780.12210241342369395No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG372540.1213726268660626No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG371920.1211706323724325No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN354090.11536166169271517No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNN352550.11485993343434361No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN342490.11158240987641001No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNN338040.11013261068825846No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN337360.10991106834040607No Hit
TTGATCATCGACACTTCGAACGCACNNNNNNNNNN337010.10979703919077616No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN336450.10961459255136835No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNNN329190.10724930219047389No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN320900.10454844033209719No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN307390.10014691515638316No Hit

[OK]Adapter Content

Adapter graph