FastQCFastQC Report
Sat 16 Sept 2023
SRR15513193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30433039
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length35-50
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN22049667.245303369144304No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN18696726.143559964550369No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN12170053.9989598146934977No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN8114072.6662043182739654No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN6530572.1458816518455484No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN5964201.9597779899667593No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5949201.9548491361641538No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN5456001.7927884231344757No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN4461201.4659068389456604No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN4211841.383969573331142No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN2850340.9365939431812905No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN2832510.9307351789612598No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN2806950.9223364120816195No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN2015520.6622802277485333No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNN1777810.5841710385873721No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN1658050.5448190698273675No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN1631510.5360982844992904No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN1548860.5089403000469326No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN1538160.5054243843344071No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN1394770.4583078278840309No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN1378850.4530766710481986No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC1350200.44366256028522155No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN1334980.4386614166268443No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN1151520.3783782487184405No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN944530.3103633521450158No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNNN934580.30709387912262065No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN808300.2655995019097501No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN786540.2584493779934367No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN726900.2388522552742761No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTNN687000.2257415041593447No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNN637140.2093579941194831No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN616140.202457598795835No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN589980.19386167776409055No Hit
ATTGATCATCGACACTTCGAACGCANNNNNNNNNN588870.1934969425826977No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN570380.18742130879535232No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCNNNNN565490.18581450245570283No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN560850.18428984367943013No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNN499150.164015825038045No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN481800.15831478413969763No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN481340.1581636326230844No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN468870.15406611216185148No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN458550.15067506074565867No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN454940.14948884993049824No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN451600.14839135848378468No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN444180.14595321880276235No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN438510.14409011206537736No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN423720.13923026221600807No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN411620.13525432014857272No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN390360.1282684913590128No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTNNNNN386600.1270329920058263No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN382820.12579092084756963No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNNN382180.12558062308532514No Hit
TCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNNN372890.12252801963024461No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN372620.12243930026179771No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNNN370310.12168025677619643No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNN367100.12062548206243878No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN365670.12015559799992369No Hit
ACGATGCCGACCGGCGATGCGGCGGCGNNNNNNNN361250.11870322907942253No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTNNNNNN357890.11759916582763884No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN344150.11308433574445194No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCNN343070.11272945827066432No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNN337630.11094192729158596No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN325970.10711056493569374No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNN323390.10626280208164554No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG318290.10458699178875959No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN310360.10198127107844865No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN309770.10178740282887949No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)