FastQCFastQC Report
Sat 16 Sept 2023
SRR15513194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30799809
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length35-50
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN19597856.362977770414095No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN15844845.144460473764626No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN14411864.6792043418191325No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13806954.482803773231192No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN9505863.086337321117803No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN7792732.530122832904581No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN6052791.9652037452569917No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN5460491.7728973579024465No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN5204661.6898351544972243No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN3823981.2415596473341766No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN3772841.2249556482639228No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN3158881.025616749766208No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN2919730.9479701643604349No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN2557680.8304207341026043No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN2503780.8129206255792041No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN2259160.7334980551340432No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN2141720.6953679485479927No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN2103830.683065924207517No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN1805210.5861107775051462No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN1685090.547110535652997No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN1555600.5050680671428839No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNN1451190.4711685062722305No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC1380000.4480547265731421No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN1378290.4474995283249971No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN1356780.4405157187825418No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1334610.4333176221969428No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNNN1187100.3854244680543311No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG1139320.3699113848400813No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN1023440.3322877749014613No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN968600.3144824696802503No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN959910.3116610236121919No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN910550.29563495020374964No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN879400.2855212511220443No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN776660.25216390140601197No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN755190.24519307895708056No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN650420.2111766342447124No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN642380.20856622844641665No Hit
ATTGATCATCGACACTTCGAACGCANNNNNNNNNN575700.18691674354214338No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG548890.17821214410777678No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN533530.17322510019461484No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN516800.16779324832826073No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN494510.1605561904620902No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN470960.1529100391499181No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNN462490.15016002209624094No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTNN461690.14990028022576374No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN447240.14520869269026962No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN436620.141760619359685No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNNN409030.13280277160160311No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG405780.13174757025278955No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN386770.12557545405557546No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGANN380400.1235072594119009No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN372460.12092932134741485No Hit
TCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNNN368010.11948450719288552No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN367930.1194585330058378No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN359030.11656890469677914No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN355990.11558188558896583No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN347660.11287732336262214No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN344830.11195848649580911No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC342560.11122146893833011No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN342350.11115328669732984No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN339360.11018250145642136No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG338540.10991626603918225No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCNN331030.1074779392300777No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNN319880.10385778691030195No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN308650.10021166040347848No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)