FastQCFastQC Report
Sat 16 Sept 2023
SRR15513195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29129210
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length35-50
%GC70

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN17085045.865260334900946No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN11652914.000420883367588No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11132833.8218784512178665No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN8083862.7751730994421067No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN7374582.5316786826693893No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN7365082.5284173515176No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN6988242.399048927176535No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN6853752.352878777007684No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN6310602.166416459629355No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN6249952.1455954349603026No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN6095022.092408273344866No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN5878002.017905737917369No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN5557371.9078340950544146No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN5027321.7258689816853943No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN4720301.6204696248198973No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN4311721.4802049214516975No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN4270431.4660301463719752No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN4016741.378938872698573No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN3351851.1506834548551095No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN3174311.089734325098415No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN3127451.0736473800696964No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN2678030.9193623857289642No Hit
ACGATGCCGACCGGCGATGCGGCGGCGNNNNNNNN2462550.8453885292460729No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN2302980.790608464836499No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN2062340.7079972302716071No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNN1934280.664034486345493No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN1426200.4896116303875045No Hit
CGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNN1312780.4506747694153051No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1294080.44425509651652073No Hit
ACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNNN1263400.4337227133863225No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN1097690.3768347991586452No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN1056620.3627355496424379No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN1033040.35464058242568197No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNN954070.327530338103917No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN759970.26089619320263063No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN733340.25175416703714243No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN665590.22849572645464808No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN658260.22597935199753102No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN656970.22553649755691968No Hit
CCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNN654860.22481213874320657No Hit
AACGATGCCGACCGGCGATGCGGCGGCNNNNNNNN642680.22063076890859726No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN620440.21299582103325151No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN583790.200413948747666No Hit
AGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNN576470.19790100727070867No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN551580.18935631965302183No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN531450.1824457305913892No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN525050.1802486232891314No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN524540.18007354130098277No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN466340.16009359677107618No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN450140.1545321689122362No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN437870.1503199022561889No Hit
ACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNNN429640.1474945595846918No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN423960.14554462685393801No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN419520.14402038366299671No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN408570.14026127038804004No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN404640.13891210918524738No Hit
AAACGATGCCGACCGGCGATGCGGCGGCNNNNNNN389210.1336150207987103No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN376300.1291830434124372No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN372700.12794717055491722No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNN357170.12261575236678234No Hit
TCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNN356230.12229305223176323No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNN341720.11731179801992571No Hit
TCTAGCATCACCAGTATTAGAGGNNNNNNNNNNNN338420.11617891456719905No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN322760.11080286763698707No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN322380.1106724143909155No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTNNNNNNNN307300.10549548030997064No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN304520.10454111182555242No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGNNNNNNN294610.10113902848721265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)