FastQCFastQC Report
Sat 16 Sept 2023
SRR15513196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30979840
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length35-50
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN25549738.247211735115481No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN17340835.597456281246126No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN14557004.698862227822998No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN11984203.868386666942115No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN10272113.315740171672933No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN6996382.2583654402346816No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6000291.9368369881832832No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN4618451.4907920763954883No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN4187671.3517403576002975No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN4026021.299561263066562No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN3364201.0859320125604264No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN3304341.0666097694500682No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN3045410.983029608932777No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN2980470.9620675897615998No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN2872970.927367604222617No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNN2854090.9212733183902823No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2115580.6828892595959178No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN1756830.5670881450646614No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN1579710.509915480518944No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1370570.4424070621410569No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN1362720.43987315622030326No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN1304930.42121908957567245No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN1281100.4135269904557286No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN1274230.41130941928686526No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN1173280.3787237119365368No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN1130430.36489213630541667No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN1087390.3509992304672974No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN1064050.34346529872329873No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN1061720.34271319671115147No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN1052030.33958535615419577No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN984690.3178486396314506No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN981240.31673501218857164No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN968260.31254519067884146No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG935650.30201899041441144No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN924210.2983262663719374No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN917160.2960505929017064No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN884080.2853726810725943No Hit
ATTGATCATCGACACTTCGAACGCANNNNNNNNNN810820.2617250444159815No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN789130.2547237171011858No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN738780.23847121224641576No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN727890.234956023013676No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN725630.2342265163409495No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN672210.21698304445729866No Hit
ATTGATCATCGACACTTCGAACGCACNNNNNNNNN670360.216385881915465No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN655190.21148914907242905No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN611720.19745744329215387No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNN591940.19107264595298104No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGANN585260.1889164049911168No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN565160.1824283146717349No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN562440.18155032433995785No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN547520.1767342891377102No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN521890.1684611670040904No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN518310.16730557678800148No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN509020.16430685245630705No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN506230.1634062667851093No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC504240.16276391356443418No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN490340.1582771247366029No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN463290.1495456400033054No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNN462980.14944557492872784No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN456930.14749269202165022No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN451980.14589487873404122No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN446240.14404206090154112No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN444730.1435546471511796No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN429880.13876120728835267No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCNN426240.13758624963847457No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNN419260.13533317150766436No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN419220.1353202598851382No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN410120.13238286576044292No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN398620.12867077428417967No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN395820.12776696070735033No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN387190.12498127814733712No Hit
ACCCCTCCCACGGCGCGACCGCNNNNNNNNNNNNN385790.12452937135892246No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTNN381690.1232059300499938No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN381070.12300579990083874No Hit
ACCCCTCCCACGGCGCGACCGCTCNNNNNNNNNNN380320.12276370697847375No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN372410.12021043362393093No Hit
TTGATCATCGACACTTCGAACGCACNNNNNNNNNN354380.11439051977027641No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG316160.1020534644465562No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNN310970.10037818142379043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)