FastQCFastQC Report
Sat 16 Sept 2023
SRR15513197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35373674
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN29822438.430684921221358No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN19221655.433885663106411No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN5882851.6630588046918735No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN5498311.5543508429460846No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN4128961.1672409261192376No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATNNNNNN3670341.0375908366204767No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3049680.8621326696231779No Hit
AGACGTGGCGACCCGCTGAATTTAAGCNNNNNNNN3011750.8514100062097028No Hit
AGATCAGACGTGGCGACCCGCTGAATTTNNNNNNN2928510.8278783820985064No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGNNN1976800.5588336682245673No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTNNNNNNNN1895570.5358702632924135No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN1746480.4937231004051205No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1669350.47191874951977003No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN1599120.45206500178635667No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCNNN1598160.4517936135217394No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN1556350.4399740892054357No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGANN1548710.43781429093285584No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTNNNN1474170.4167421229697543No Hit
AAGGCTAAATACCGGCACGAGACCGATNNNNNNNN1370620.387468940885247No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACNN1295520.3662384631011186No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGANN1180430.3337029679190236No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN1161800.3284363394087931No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTNNNNNNNNN1057080.2988324028767835No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN1024790.28970414551793516No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN1024700.2896787028681273No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN1012020.2860941162063064No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTNNNNN910790.2574767890946244No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN852470.2409899520191202No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN845410.23899411748974675No Hit
AGCCCGTGGACGGTGTGAGGCCGGTNNNNNNNNNN813650.230015689068656No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN794550.22461619338720656No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN787760.22269668680725674No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN769640.21757423331260414No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNN741000.20947781675151977No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATNNNNN726580.20540133886007994No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGANNN718320.2030662689999348No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGT693460.19603844373078128No Hit
AATCATGGCCTCAGTTCCGAAAACCAACNNNNNNN692420.1957444397774458No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTNNNNNNNN679390.19206090947748317No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN675720.19102341475753976No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN674630.19071527599875546No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNN669970.18939791213092538No Hit
AGCTGCGAGAATTAATGTGAATTGCAGNNNNNNNN669830.18935833467566868No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNN669600.18929331457060414No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATNNNNNN647060.18292134427427584No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCNNNNNN624920.17666245242153814No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNN616650.17432455560030322No Hit
AGCTGCGAGAATTAATGTGAATTGCNNNNNNNNNN602370.17028765516412006No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN594350.16802043237012926No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCNNNNNNN590690.1669857646112756No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCNNNNNNN580480.16409943733862647No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATATTNNN575740.1627594577820783No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCNNNNN572780.1619226772995081No Hit
AGCGACGGGCGGTGTGTACAAAGGGCNNNNNNNNN567270.1603650217390481No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGNNNNNNN563080.15918052504243693No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCNNNN557390.15757198418236115No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTNNNN553820.15656275907331538No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCNNNNNNNN538940.15235624097174638No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN534140.15099929964865963No Hit
AGATCAGACGTGGCGACCCGCTGAATTNNNNNNNN525630.14859355576127037No Hit
AAGAGCATCGAGGGGGCGCCGAGAGGCNNNNNNNN517680.1463461216949079No Hit
ACTCGGCTCGTGCGTCGATGAAGAACGCNNNNNNN517570.1463150251229205No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN514660.14549237944579915No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTNNNN511170.1445057700254715No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCC506550.14319971400200046No Hit
ATCCGGTAAAGCGAATGATTAGAGGTNNNNNNNNN503720.14239968401359723No Hit
AAATGGGTAAGAAGCCCGGCTCGCTGGCGTNNNNN501820.14186256140654205No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTNN498600.14095227993563803No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCNNNNNNN481920.1362369088379115No Hit
AGCGGTGGATCACTCGGCTCGTGCGTCGATNNNNN476730.1347697160323239No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACNN472700.133630450713149No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN470230.13293219132397727No Hit
ACTTCGAACGCACTTGCGGCCCCGGGNNNNNNNNN466660.1319229662149315No Hit
AGTGTAGCGCGCGTGCAGCCCCGGACNNNNNNNNN460640.13022113563889348No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN453720.12826487856477672No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN423600.11975007176240726No Hit
TTTATCCGGTAAAGCGAATGATTAGAGGTNNNNNN422920.11955783840830331No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNN411740.11639729590994705No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN396460.1120776993647875No Hit
ACAAAGGGCAGGGACTTAATCAACGCNNNNNNNNN396190.11200137141536386No Hit
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCNNNN395990.11194483219356859No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTNNNNNNNNN389000.10996878639182348No Hit
AGCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGAC388170.10973414862137307No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCNNN379060.10715878706859797No Hit
AATCGGTAGTAGCGACGGGCGGTGTGTACNNNNNN367620.10392474358190784No Hit
GCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACNN362020.10234164537163994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)